大鼠肾低温损伤特点及机制研究  

Characteristics and mechanisms of renal hypothermia injury in rats

作　　者：窦建平 王宁[1] 刘丽丽[1] 李军[1] DOU Jian-Ping;WANG Ning;LIU Li-li;LI Jun(The Sixth Medical Center,Chinese PLA General Hospital,Beijing 100048,China)

机构地区：[1]解放军总医院第六医学中心,北京100048

出　　处：《军事医学》2023年第4期289-292,共4页Military Medical Sciences

基　　金：装备军内科研综合论证项目(LB20211A010016)。

摘　　要：目的 观察不同低温条件下大鼠肾结构、功能以及细胞凋亡的变化特征。方法 成年雄性SD大鼠,随机分为3组:对照组(C组)、轻度低温组(MH组)和重度低温组(SH组),每组8只。利用低温水槽浸泡身体将体温降至目标体温后维持约60 min。降温后12 h抽取静脉血分离血清,观察各组肾功能,包括肌酐(Cr)、血尿素氮(BUN)以及尿酸(UA)改变;利用过碘酸雪夫染色(PAS)观察肾损伤病理特征,给予末端脱氧核苷酸转移酶介导的dUTP生物素缺口末端标记(TUNEL)染色观察肾小管以及肾小球等细胞凋亡。结果 和对照组相比,降温后12 h,轻度低温组仅有UA水平升高,重度低温组Cr、BUN和UA均有不同程度升高,其结果具有统计学意义(P<0.001)。PAS染色显示,轻度低温会导致肾小管损伤,上皮细胞排列不齐,重度低温损伤进一步加重。TUNEL染色显示,重度低温组大鼠肾小管细胞凋亡明显增多。结论 重度低温可导致大鼠肾功能减退、肾结构损伤,尤其是肾小管细胞凋亡增加。Objective To observe the characteristics of changes of renal structure,function and apoptosis in rats under different hypothermic conditions.Methods Twenty-four adult male Sprague-Dawley(SD)rats were randomly divided into three groups:the control group(group C),mild hypothermia group(group MH)and severe hypothermia group(group SH)(n=8 each).The animal body temperature was reduced to the target one using a low-temperature water chamber and was maintained for about 60 minutes.Twelve hours later,blood serum was separated from the venous blood of animals in each group to assay the indicators of renal function,including serum creatinine(Cr),blood urea nitrogen(BUN),and uric acid(UA).Periodic acid schiff(PAS)staining was used to observe the pathological changes of kidney injury,while terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL)staining was used to observe the cell apoptosis of renal tubules and glomeruli.Results The serum UA level was significantly increased in rats of group MH at 12 hours after operation compared with the control group,while concentrations of serum Cr,BUN and UA were markedly increased in rats of group SH,there were statistically significant differences(P0.001).PAS staining showed that mild hypothermia could lead to renal tubular injury and disorder of epithelial cells,especially in group SH.TUNEL staining showed that renal tubular cell apoptosis was significantly aggravated in rats with severe hypothermia.Conclusion Severe hypothermia can aggravate renal dysfunction,structural damage and apoptosis of the kidney.

关 键 词：大鼠 低温 急性肾损伤 肾功能 细胞凋亡 

分 类 号：R692[医药卫生—泌尿科学]