肿瘤细胞表面伞形胶束诱导特异性识别荧光增强与数字信号放大的单细胞轮廓可视化  

Visualization of Single Cell Contour by Umbrella-Shaped Micelle on the Surface of Tumor Cell Induced Specific Recogonized Fluorescence Enhancement and Digital Signal Amplification

作　　者：李小魏 曾启[2,3] 张智娴 符晨 任兆刚 谢洪平[2] LI Xiaowei;ZENG Qi;ZHANG Zhixian;FU Chen;REN Zhaogang;XIE Hongping(College of Environmental Science and Engineering,China West Normal University,Nanchong 637002,China;College of Pharmaceutical Sciences,Soochow University,Suzhou 215123,China;Dushuhu Hospital Affiliated to Soochow University,Suzhou 215123,China)

机构地区：[1]西华师范大学环境科学与工程学院,南充637002 [2]苏州大学药学院,苏州215123 [3]苏州大学附属独墅湖医院,苏州215123

出　　处：《理化检验（化学分册）》2023年第4期453-460,共8页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：以循环肿瘤细胞(CTC)的特异性膜蛋白EpCAM为识别受体,以羧基荧光素(FAM)标记的EpCAM核酸适体(Apt-FAM)为识别配体,当Apt-FAM特异性识别EpCAM后,细胞表面分布着大量的FAM荧光团,FAM荧光团的疏水性诱导非离子型表面活性剂Tween-80的疏水端在FAM周围聚积,形成以FAM为中心的伞形纳米胶束,从而增强了细胞膜的特异性识别荧光,提高了荧光信号的信噪比;利用数字信号放大技术,将成像细胞的膜荧光信号放大,使成像细胞的轮廓完整、清晰地呈现出来,实现了单细胞特异性识别轮廓的可视化,提高了捕获CTC计数和尺度估计的准确度。The specific membrane protein EpCAM of circulating tumor cell(CTC)was used as the recognition receptor,and the carboxyfluorescein(FAM)-labeled EpCAM aptamer(Apt-FAM)was used as the recognition ligand.After Apt-FAM specifically recognized EpCAM,the fluorophore of FAM was distributed abundantly on the surface of cell.The hydrophobicity of the fluorophore of FAM induced the accumulation of the hydrophobic end of the non-ionic surfactant Tween-80 around the fluorophore to form an umbrella-shaped nanomicelle centered on FAM,which enhanced the specific recognized fluorescence on the surface of cell and improved the signal-to-noise ratio of the fluorescence signal.Using digital signal amplification technology,the membrane fluorescence signal of the imaging cells was amplified,and the contours of the imaging cells were completely and clearly displayed,which realized the visualization of the contours of single cells by specific recognition,and improved the accuracy of count and scale estimation of capturing CTC.

关 键 词：循环肿瘤细胞 特异性识别 荧光增强 可视化 数字信号放大 

分 类 号：O652.3[理学—分析化学]