梯度液配制时间对精液优化处理回收率影响的研究  

Effect of gradient solution standing time on recovery rate of semen optimal treatment

作　　者：梁仲城[1] 覃晓[1] 余飞[1] 黄艳云 覃卫玲 覃永长 Liang Zhongcheng;Qin Xiao;Yu Fei;Huang Yanyun;Qin Weiling;Qin Yongchang(Department of Reproductive Center,the People's Hospital of Baise,Baise 533000 Guangxi,China)

机构地区：[1]百色市人民医院生殖医学中心,广西百色533000

出　　处：《中国男科学杂志》2023年第2期42-45,共4页Chinese Journal of Andrology

摘　　要：目的 评价密度梯度液配制时间对不连续密度梯度离心法精液优化处理回收率的影响。方法 收集30例患者精液,按实验设计方案制备1.5 mL 40%+1.5 mL80%SpermGrad双层梯度液,分别静置2 h、1 h、30 min、20 min、10 min、5 min后,各管同时加入1 mL同一病人精液进行密度梯度离心精液优化处理,处理后计算各自精液前向的(progressive, PR)精子回收率并统计比较。结果 配制后静置2 h、1 h、30 min、20 min、10 min、5 min精液PR回收率分别为(37.21±3.89)%、(39.62±4.30)%、(43.09±4.11)%、(45.89±4.71)%、(46.65±4.65)%、(46.87±4.84)%。配制20 min、10 min PR回收率与5 min比较无显著差异(P>0.05),配制时间大于20 min各组PR回收率与5 min组比较有显著差异(P<0.05)。结论 梯度液配制时间>20 min PR回收率有显著下降,建议梯度液配制后20 min内进行梯度离心以提高PR回收率。Objective To analyze the effect of the standing time of density gradient solution on the recovery rate of semen optimized by discontinuous density gradient centrifugation.Methods Semen samples from 30 patients were collected.According to the experimental design,SpermGrad bilayer gradient solution(1.5 mL of 40%+1.5 mL 80%)was prepared,and then placed for 2 hours,1 hour,30 minutes,20 minutes,10 minutes,and 5 minutes,respectively.Afterwards,1 mL of the same patient's semen was simultaneously transferred to the gradient solution mentioned above for optimization centrifugation treatment,and their PR recovery rates were calculated and compared statistically.ResultsThe PR recovery rates of semen were(37.21±3.89)%、(39.62±4.30)%、(43.09±4.11)%、(45.89±4.71)%、(46.65±4.65)%、(46.87±4.84)%,after the gradient solutions were placed for 2 hours,1 hour,30 minutes,20 minutes,10 minutes,and 5 minutes.There was no significant difference in PR recovery rate between 20min,10 min and 5 min(P>0.05),However,the PR recovery rates of the groups with the standing time over 20 minutes were significantly different from that of the group with standing time for 5 min(P<0.05).Conclusion The PR recovery rate is significantly decreased when the standing time of gradient solution is more than 20 minutes.It is suggested to perform gradient centrifugation within 20 minutes after the gradient solution preparation for improving the PR recovery rate.

关 键 词：授精 人工(丈夫供体) 离心法 梯密度 精液优化处理 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]