细菌非编码小RNA的生物学特征及作用机制研究进展  

Biological Characteristics and Regulation Mechanisms of Bacterial Non-coding Small RNA

作　　者：王海燕[1] 贺婷停 李昊阳 WANG Haiyan;HE Tingting;LI Haoyang(College of Life Sciences,Sichuan University,Chengdu 610064,Sichuan)

机构地区：[1]四川大学生命科学学院,四川成都610064

出　　处：《四川师范大学学报（自然科学版）》2023年第5期581-590,F0002,共11页Journal of Sichuan Normal University（Natural Science）

基　　金：国家自然科学基金(32060720);四川省科技计划(2019YFG0273)。

摘　　要：细菌非编码小RNA(sRNA)是一类长度为50~500 nt、一般不编码蛋白质的具有调控功能的小RNA,主要通过与靶mRNA碱基配对或与靶蛋白质结合,在转录后水平发挥调控作用.sRNA参与细菌氨基酸代谢和转运、环境胁迫响应以及毒力作用等众多重要生理过程的调控,与传统的转录因子等构成多层级的调控网络,协同作用使细菌对环境变化做出快速且精细的响应.由于sRNA在细菌调控网络中的重要作用,发现新的sRNA并阐明它们的调控功能成为原核生物基因表达调控研究的一个热点.本综述对细菌sRNA的筛选、鉴定、作用机制以及生物学功能等方面取得的进展进行总结,希望给相关领域的研究者提供一个较为全面的视角.Bacterial non-coding small RNAs(sRNA)exist as relatively short transcripts(50-500 nt)that are transcribed but do not encode proteins.sRNA plays a post-transcriptional regulatory role on target genes mostly by imperfect base-pairing with target mRNA,and rarely by binding target protein.sRNA is involved in the regulation of many important physiological processes,such as amino acid metabolism and transport,stress response and virulence,etc.,forming a multi-level regulatory network with traditional transcription factors to enable bacteria responding quickly to environmental stress.Due to the important role of sRNA in bacterial regulatory network,the discovery of new sRNAs and revealing their regulatory mechanisms have become a hotspot in prokaryotic gene expression regulation research.This review summarizes the advance in screening,identification,regulatory mechanism and biological function of bacterial sRNA,hoping to provide a more comprehensive perspective for researchers of related fields.

关 键 词：细菌 非编码sRNA 转录后调控 

分 类 号：Q939.1[生物学—微生物学]