原位调控基料碳源组分对水虻堆肥效率与碳氮固定效应的影响  

Effect of the black soldier fly larvae composting efficiency and carbon-nitrogen fixation by in-situ regulation of carbon source composition

作　　者：严业皓 林嘉聪 麦力文 朱四喜 李勤奋[2,3] 王定美 YAN Ye-hao;LIN Jia-cong;MAI Li-wen;ZHU Si-xi;LI Qin-fen;WANG Ding-mei(School of Ecological Environment Engineering,Guizhou Minzu University,Guiyang 550025,China;Institute of Environment and Plant Protection,Chinese Academy of Tropical Agricultural Sciences,Key Laboratory of Low-carbon Green Agriculture in Tropical Region of China,Ministry of Agriculture and Rural Affairs,P.R.China,Hainan Provincial Key Laboratory of Tropical Ecological Circular Agriculture,Haikou 571101,China;Hainan Danzhou Tropical Agro-ecosystem National Observation and Research Station,National Agricultural Experimental Station for Agricultural Environment,National Long-term Experimental Station for Agriculture Green Development,Danzhou 571737,China)

机构地区：[1]贵州民族大学生态环境工程学院,贵州贵阳550025 [2]中国热带农业科学院环境与植物保护研究所,农业农村部热区高效农业绿色低碳重点实验室,海南省热带生态循环农业重点实验室,海南海口571101 [3]海南儋州热带农业生态系统国家野外科学观测研究站,国家农业环境儋州观测实验站,国家农业绿色发展长期固定观测儋州试验站,海南儋州571737

出　　处：《中国环境科学》2023年第6期2970-2981,共12页China Environmental Science

基　　金：海南省自然科学基金青年基金项目(421QN295);海南省重大科技计划项目(ZDKJ2021009);中央级公益性科研院所基本科研业务费专项(1630042021019,1630042022002)。

摘　　要：选择生物可利用度高的葡萄糖和生物利用度低的木屑作为碳源,花生粕作为氮源,配制总C/N为10、15、20、25、30,碳源难易利用程度为0%、25%、50%、75%、100%的25个水虻堆肥处理组,探究了不同总碳氮比和碳源难易利用程度下的水虻堆肥效率与碳氮素固定效应,基于相关性分析与正交偏最小二乘回归模型分析了调控碳氮固持的关键因子,并利用主成分分析法开展综合评价.结果表明:25个试验处理下,水虻虫体和虫粪产率分别为3.96%~23.06%和13.38%~89.99%;虫体碳含量为48.12%~60.31%,虫体氮含量为5.79%~9.85%,水虻从基料回收的碳、氮素分别为3.24%~29.58%和17.21%~52.69%;水虻堆肥体系碳与氮总固定率分别为40.69%~93.81%和38.06%~72.89%.易利用碳素有效率(ACC1)对水虻堆肥中虫体、虫粪的碳氮固定影响显著(VIP>1).总C/N为10,难易利用度碳源比为50%:50%时,水虻堆肥效率与体系总碳氮固定综合效果最佳.本研究证明了总C/N作为水虻堆肥营养基料配置指标上存在局限性,而原位调控基料碳源的生物利用度,可实现虫体高效生产与碳氮固定效能的协同平衡.The glucose and rubber sawdust which is considered as high bioavailable and low bioavailable material,respectively,was set as carbon source.And the peanuts meal was used as the nitrogen source in the BSFL composting.25composting treatments with C/N of 10、15、20、25、30,and the ratio of available carbon component in 0%、25%、50%、75%、100%.Orthogonal partial least squares regression and relevance analysis were used to determine the key indices of composting and C-N fixation effect.BSFL composting effect between different treatments were comprehensively evaluated by principal component analysis.The results indicated that productivity of the larva and larva-cast were 3.96%~23.06%and 13.38%~89.99%,respectively.The content of C and N in larva of was 48.12%~60.31%and 5.79%~9.85%,respectively,of which 3.24%~29.58%and 17.21%~52.69%were recovered from the composting substrate,respectively.The total C and N fixation rates in BSLF composting were 40.69%~93.81%and 38.06%~72.89%,respectively.The proportion of carbon availability and active nitrogen components exhibits a significant effect on the C-N conversion rate of larva and larva cast(VIP>1).The ACC1(Available Activity Carbon 1)content and proportion should be more focus on in practical BSFL composting.The best comprehensive effect larva quality and C-N fixation were obtained in the total C/N of 10with the ratio of available to unavailable carbon component in 1:1.It is insufficient to conduct the BSFL composting only relying on substrate C/N without considering their availability.This study is significant for providing an efficient in-suit method in accurately regulating the bioavailability of the carbon source of the substrate,and finally result in enhancement of larva conversion efficiency and the C-N fixation.

关 键 词：黑水虻 堆肥 碳氮比 生物利用度 碳氮固定 

分 类 号：X705[环境科学与工程—环境工程]