枸杞棉蚜对吡虫啉的抗药性监测及交互抗性测定  

Drug resistance monitoring of Aphis gossypii Glover to imidacloprid and determination of cross resistance spectrum

作　　者：张慧斌 邵海南 黄铮昱 李秋荣[1] ZHANG Huibin;SHAO Hainan;HUANG Zhengyu;LI Qiurong(Academy of Agriculture and Forestry Sciences,Scientific Observing and Experimental Station of Crop Pest in Xining,Ministry of Agriculture and Rural Affairs,Key Laboratory of Agricultural Integrated Pest Management,Xining 810016,China;State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016,China;College of Food Science and Engineering,Central South University of Forestry and Technology,Changsha 410004,China)

机构地区：[1]青海大学农林科学院,农业农村部西宁作物有害生物科学观测实验站,青海省农业有害生物综合治理重点实验室,青海西宁810016 [2]青海大学三江源生态和高原农牧业国家重点实验室,青海西宁810016 [3]中南林业科技大学食品科学与工程学院,湖南长沙410004

出　　处：《青海大学学报》2023年第3期44-51,共8页Journal of Qinghai University

基　　金：青海省科学技术厅项目(2017-ZJ-934Q);湖南省教育厅科学研究项目(19B597)。

摘　　要：为了解田间枸杞棉蚜对吡虫啉抗药性的发生动态,延长吡虫啉的使用寿命,文中采用单头反选育和群体汰选的方法,建立了枸杞棉蚜吡虫啉敏感品系和抗性品系,对抗性品系现实遗传力与抗性风险进行评估,通过浸叶法测定11种杀虫剂对吡虫啉的交互抗性,并对青海5个县(市)的枸杞棉蚜种群进行了连续2年的抗药性监测。结果显示:初始枸杞棉蚜种群经42代筛选后,敏感种群的抗药性为原始种群的0.49倍,抗性种群的抗药性为原始种群的46.22倍,为敏感种群的92.78倍,达到高水平抗药性;枸杞棉蚜对吡虫啉的现实遗传力为0.044 7。交互抗性测定表明,吡虫啉与吡蚜酮、高效氯氰菊酯、毒死蜱、螺虫乙酯、苦参碱、鱼藤酮、除虫菊素等杀虫剂之间无交互抗性,与啶虫脒、阿维菌素、烯啶虫胺等杀虫剂的交互抗性较高。2020年和2021年5个枸杞棉蚜种群对吡虫啉均呈现中水平的抗药性,因此,长期大量使用吡虫啉存在枸杞棉蚜对吡虫啉产生抗药性的风险。In order to find out the dynamics of drug resistance to imidacloprid in Aphis gossypii Glover and prolong the field life of imidacloprid,imidacloprid-sensitive strains and imidacloprid-resistant strains of Aphis gossypii Glover are established by the methods of single head reverse breeding and mass elimination in the laboratory.The actual heritability and resistance risk of the strains are evaluated as well.The cross resistance of 11 types of insecticides to imidacloprid is determined by leaf soaking method.The resistance of Aphis gossypi Glover populations in five counties(cities)of Qinghai is monitored for two consecutive years.The results show that after 42 generations of screening for the original Aphis gossypii Glover,the drug resistance of the sensitive population is 0.49times that of the original population,the drug resistance of the resistant population is 46.22 times that of the original one and 92.78 times that of the sensitive one,reaching a high level of drug resistance.The actual heritability to imidacloprid is 0.0447.According to the test results,there is no cross resistance between imidacloprid and insecticides such as pymetrozine,beta-cypermethrin,chlorpyrifos,spirotetramat,matrine,rotenone and pyrethrin,while the cross resistance between imidacloprid and insecticides such as acetamiprid,avermectin and nitenpyram is high.The five populations show moderate drug resistance to imidacloprid in 2020 and 2021.Aphis gossypi Glover will develop resistance to imidacloprid after long-term use of imidacloprid in large quantities.

关 键 词：枸杞棉蚜 抗药性 吡虫啉 敏感种群 抗性种群 

分 类 号：S435.622[农业科学—农业昆虫与害虫防治]