环介导等温扩增技术在植物寄生线虫检测中的应用  

Application of Loop-mediated Isothermal Amplification Technology in Detecting Plant Parasitic Nematode

作　　者：王一椒 WANG Yijiao(Shanghai Chenshan Botanical Garden,Shanghai 201602)

机构地区：[1]上海辰山植物园,上海201602

出　　处：《现代农业科技》2023年第11期105-109,共5页Modern Agricultural Science and Technology

基　　金：上海市绿化和市容管理局科研专项(G202406)。

摘　　要：植物寄生线虫是世界各地农林业生产中一种重要的生物胁迫,在早期快速、准确地鉴定出植物寄生线虫种类是减少其危害的重要前提。环介导等温扩增(LAMP)技术因在实际操作中具有简易、快速和灵敏等特点,已被广泛应用于多种植物病原体的检测和诊断。本文主要就LAMP技术在伞滑刃线虫、根结线虫、孢囊线虫、短体线虫、滑刃线虫、粒线虫等重要植物寄生线虫检测中的应用研究情况进行了综述,以期为针对不同的植物寄生线虫开发和选用最佳的LAMP检测方法提供参考。Plant parasitic nematode is an important biological stress in agricultural and forestry production around the world.Rapid and accurate identification of plant parasitic nematode species in the early stages is an important prerequisite to reduce their harm.Loop-mediated isothermal amplification(LAMP)technology has been widely used in the detection and diagnosis of many plant pathogens due to the advantages of simpleness,quickness and acuteness in operation.This paper reviewed the research progress on the application of LAMP in important plant parasitic nematodes,such as Bursaphelenchus,Meloidogyne,Heterodera,Pratylenchus,Aphelenchoides and Anguina,in order to provide a reference for developing and selecting the optimal LAMP detection method for different plant parasitic nematodes.

关 键 词：环介导等温扩增(LAMP)技术 植物寄生线虫 检测 

分 类 号：S433[农业科学—农业昆虫与害虫防治]