分化拮抗非编码RNA-201对人脐静脉内皮细胞增殖的影响  

Effect of Differentiation Antagonizing Non-protein Coding RNA-201 on the Proliferation of Human Umbilical Vein Endothelial Cells

作　　者：阿达莱提·卡米力 陈宇[1] 张伟丽 Adalaiti·Kamili;CHEN Yu;ZHANG Weili(Fuwai Hospital,Chinese Academy of Medical Sciences&Peking Union Medical College,National Center for Cardiovascular Diseases,National Clinical Research Center for Cardiovascular Diseases,State Key Laboratory of Cardiovascular Diseases,Beijing 100037,China)

机构地区：[1]中国医学科学院、北京协和医学院、阜外医院、国家心血管病中心、国家心血管疾病临床医学研究中心、心血管疾病国家重点实验室,北京市100037

出　　处：《中国分子心脏病学杂志》2023年第2期5333-5338,共6页Molecular Cardiology of China

基　　金：国家自然科学基金(82070373)。

摘　　要：目的探讨分化拮抗非编码RNA(DANCR)-201对人脐静脉内皮细胞(HUVECs)增殖的影响。方法分离培养HUVECs后,构建过表达或敲低DANCR-201的细胞模型,采用长时间动态活细胞成像方法和细胞划痕实验检测HUVECs增殖和迁移能力。采用实时荧光定量PCR和蛋白质印迹法检测细胞增殖相关蛋白Phospho-c-Raf和细胞周期蛋白p16、p53的表达。结果过表达DANCR-201可促进HUVECs增殖[(67.1±5.4)%比(90.4±3.5)%,P<0.01]和迁移[(64.2±1.5)%比(88.6±3.1)%,P<0.05],同时细胞增殖相关蛋白Phospho-c-Raf表达水平升高(P<0.01),细胞周期蛋白p16、p53表达水平均降低(P<0.01或0.05)。敲低DANCR-201对HUVECs增殖和迁移无影响,但细胞周期蛋白p16、p53表达水平均升高(P<0.01或0.05)。结论DANCR-201促进HUVECs增殖,但其在动脉粥样硬化斑块形成中的作用机制仍需深入研究。Objective To study the role of differentiation antagonizing non-protein coding RNA(DANCR)-201 in regulating the proliferation of human umbilical vein endothelial cells(HUVECs).Methods HUVECs were isolated and cultured.The DANCR-201 over-expression or silencing cell line of HUVECs were established in vitro,and live content kinetic imaging system and wound scratching assay were used to evaluate the proliferation and migration of HUVECs.Furthermore,the expression of cyclin p16,p53 and proliferation related protein Phospho-c-Raf were examined by realtime quantitative PCR and Western blot.Results The overexpression of DANCR-201 promoted endothelial proliferation([67.1±5.4]%vs[90.4±3.5]%,P<0.01)and migration([64.2±1.5]%vs[88.6±3.1]%,P<0.05),the expression of Phosphoc-Raf was increased by 1.3 fold(P<0.01),the expression of cyclin p16 were decreased by 35%(P<0.01)and p53 were decreased by 21%(P<0.05).Conversely,silencing of DANCR-201 had no significant effect on the proliferation and migration of HUVECs,and the expression of cyclin p16 and p53 were increased by 1.6 fold(P<0.01)and 1.3 fold(P<0.05),respectively.Conclusions DANCR-201 may promote the proliferation of HUVECs,but future studies are needed to explore the related molecular mechanism of DANCR-201 on the modification of atherosclerotic plaques.

关 键 词：分化拮抗非编码RNA-201 细胞增殖 细胞迁移 人脐静脉内皮细胞 

分 类 号：R543.5[医药卫生—心血管疾病]