低强度脉冲超声波治疗冻结肩模型兔的实验研究  

Experimental study on the treatment of frozen shoulder model Rabbit with low-intensity pulsed ultrasound

作　　者：蒋梦洁 钱治军 徐思 梁伟 Mengjie Jiang;Zhijun Qian;Si Xu;Wei Liang(Department of Clinical Laboratory,Changhai Hospital,Naval Medical University,Shanghai 200043,China;Department of Medical Affarir,Changhai Hospital,Naval Medical University,Shanghai 200043,China;Department of Pathology,Changhai Hospital,Naval Medical University,Shanghai 200043,China;Department of Orthopaedic Trauma,Changhai Hospital,Naval Medical University,Shanghai 200043,China)

机构地区：[1]海军军医大学长海医院检验科,上海200043 [2]海军军医大学长海医院医务处,上海200043 [3]海军军医大学长海医院病理科,上海200043 [4]海军军医大学长海医院创伤骨科,上海200043

出　　处：《中华肩肘外科电子杂志》2023年第1期30-34,共5页Chinese Journal of Shoulder and Elbow(Electronic Edition)

基　　金：虹口区卫健委重点科研基金(虹卫1702-10)。

摘　　要：目的探讨低强度脉冲超声波(low-intensity pulsed ultrasound,LIPUS)对冻结肩模型兔的疗效和机制。方法将60只雌性新西兰兔随机分为空白对照组、模型组和LIPUS治疗组。采用持续劳损并且冰敷的方法制作冻结肩模型后,观察患肩周围组织的病理变化,并检测血清中的转化生长因子-β1(transforming growth factor-β,TGF-β1)、前列腺素-E2(prostaglandin E2,PGE-2)及羟脯氨酸(hydroxyproline,HYP)的含量。结果采用多组资料的单项方差分析方法进行统计分析,发现LIPUS治疗组中TGF-β1、PGE-2、HYP含量较模型组明显降低,而模型组中含量较空白对照组明显增高,差异均有统计学意义(P<0.05)。病理切片结果显示治疗组患肩滑膜增生和肌腱纤维增生程度改善,组织修复加速。结论LIPUS通过降低新西兰兔冻结肩周围组织中TGF-β1、PGE-2、HYP含量,达到抗炎镇痛效果,值得在临床中推广使用。Background Diffuse shoulder pain and shoulder joint mobility disorder are the main clinical manifestations of a frozen shoulder.It often occurs on the shoulder of middle-aged and older adults.The lesion involves the shoulder,joint bursae,and other soft tissues.The incidence of frozen shoulder is 2%-5%,but its pathogenesis is unknown,and there are many limitations and poor curative effects in current treatment methods.Therefore,further understanding its pathogenesis and exploring new therapies to improve the quality of life of patients with frozen shoulders is of great significance.Animal and clinical trials have demonstrated that low-intensity pulsed ultrasound(LIPUS)can accelerate fracture healing and promote the repair of other connective tissue injuries,such as muscles,tendons,cartilage,ligaments,etc.So far,no reports have been found about the effect of LIPUS on frozen shoulder animal models.Objective To investigate the effect and mechanism of LIPUS on frozen shoulder model rabbits.Methods Sixty female New Zealand rabbits were randomly divided into a control group,a model group,and a LIPUS treatment group.After the frozen shoulder model was made by the method of continuous strain and ice compress,the pathological changes of the tissues around the affected shoulder were observed,and the contents of transforming growth factor-β1(TGF-β1),prostaglandin-E2(PGE-2)and hydroxyproline(HYP)in serum were detected.Results With a single variance analysis of multiple data groups,it was found that the contents of TGF-β1,PGE-2,and HYP in the LIPUS treatment group were significantly decreased compared with the model group.In contrast,the contents in the model group were significantly increased compared with the blank control group,with statistically significant differences(P<0.05).The results of the pathological section showed that the degree of synovial hyperplasia and tendon fiber hyperplasia were improved in the treatment group,and the tissue repair was accelerated.Conclusion LIPUS can achieve anti-inflammatory and analg

关 键 词：低强度脉冲超声波 冻结肩 转化生长因子-Β1 前列腺素-E2 羟脯氨酸 

分 类 号：R684[医药卫生—骨科学]