水稻OsGLYI11.2基因启动子的克隆及表达分析  

Cloning and Expression Analysis of OsGLYIl1.2 Gene Promoter from Oryza sativa

作　　者：刘圣杰 赖剑云 刘文华 刘勤坚 陈沛 张文虎 朱庆锋 肖应辉[2] 刘军 Liu Shengjie;Lai Jianyun;Liu Wenhua;Liu Qinjian;Chen Pei;Zhang Wenhu;Zhu Qingfeng;Xiao Yinghui;Liu Jun(Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization,Agro-biological Gene Research Center,Guangdong Academy of Agricultural Sciences,Guangzhou,510640;College of Agronomy,Hunan Agricultural University,Changsha,410128)

机构地区：[1]广东省农业科学院农业生物基因研究中心,广东省农作物种质资源保存与利用重点实验室,广州510640 [2]湖南农业大学农学院,长沙410128

出　　处：《分子植物育种》2023年第11期3481-3487,共7页Molecular Plant Breeding

基　　金：广东省农作物种质资源保存与利用重点实验室项目(2020B121201008);国家自然科学基金项目(31871716);广东省公益研究与能力建设专项(2015A020209077);广东省农业科学院院长基金项目(201615)共同资助。

摘　　要：启动子对基因时空表达的调控具有关键作用。本研究利用PCR克隆了一个水稻乙二醛酶(Glyoxalase)基因Os GLYI11.2的启动子区域片段(GenBank:AB017042.1),利用PlantCARE对该片段进行了顺式作用元件分析;构建了pOsGLYI11.2∷GUS表达载体并通过农杆菌介导转入水稻中,通过检测转基因植株中GUS基因在不同器官中的表达,分析了启动子的表达调控模式。结果显示:该启动子含有调控基因表达的7类调控顺式元件,能驱动GUS基因在水稻不同组织中(胚,胚芽鞘,花)表达。说明本研究所克隆的OsGLYI11.2基因上游2 120 bp的DNA片段具有启动子活性,能够驱动报告基因的表达。本研究结果可为进一步研究OsGLYI11.2基因在水稻中的功能及其相关调控机制提供基础。Promoter plays a key role in regulation of gene expression in time and space.This study cloned a rice Gly(Glyoxalase)OsGLYI11.2 gene promoter region fragment(GenBank:AB017042.1)by polymerase chain reaction(PCR),using PlantCARE analysis of the cis-acting elements.The vector pOsGLYIl1.2::GUS was constructed and transformed into rice callus via Agrobacterium-mediated genetic transformation.The expression of GUS gene in different organs of transgenic plants was detected and the regulation mode of promoter expression was analyzed.The results showed that the promoter contained 7 category cis-regulating elements and could drive the expression of GUS gene in different tissues(embryo,coleoptile,flowers)of rice.The cloned 2120 bp DNA fragment in the upstream of OsGLYI11.2 gene possessed promotor activity and drove the effective expression of the reporter gene.The results ofthis study might provide a basis for further study on the function of OsGLYIl1.2 gene promoter and its relative regulatory mechanisms.

关 键 词：启动子 基因克隆 组织特异性表达 OsGLYI11.2 水稻 

分 类 号：S511[农业科学—作物学]