林木原生质体细胞壁再生研究进展  

Research Progress on Regeneration of Cell Wall from Forest Tree Protoplasts

作　　者：张千悦 魏文桃 张航晓 顾连峰 Zhang Qianyue;Wei Wentao;Zhang Hangxiao;Gu Lianfeng(Basic Forestry and Proteomics Research Center,College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,350002;Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology,College of Forestry,Fujian Agriculture and Forestry University,Fuzhou,350002)

机构地区：[1]福建农林大学林学院,基础林学与蛋白质组学中心,福州350002 [2]福建农林大学林学院,福建省海峡植物应用系统生物学重点实验室,福州350002

出　　处：《分子植物育种》2023年第11期3719-3732,共14页Molecular Plant Breeding

基　　金：大学生创新项目(202010389139);国家自然科学基金项目(31800566);福建省自然科学基金项目(2018J-01608);福建省科技创新团队项目(118/KLA18069A);福建农林大学杰出青年科研人才计划项目(自然科学类)(xjq202017)共同资助。

摘　　要：原生质体细胞壁再生不仅在细胞全能性的研究中具有重要作用,同时在研究核质基因重组和体细胞胚胎发生等方面具有重要的应用。因此本研究从植物原生质体的分离、纯化入手对原生质体细胞壁再生研究进展进行了综述,详细阐述了制备、培养及植株再生过程的研究进展,分析了影响原生质体培养再生植株的相关因素,此外,还对原生质体培养的方法、方式以及原生质体再生细胞壁过程中细胞的生理生化变化进行了概述,总结了原生质体再生细胞壁得到再生植株的应用。最后对今后的应用前景进行展望,为植物原生质体细胞壁再生得到再生植株技术的发展提供参考。Cell wall regeneration in plant protoplast not only plays an important role in the study of cell pluri-potency,but also has important applications in the study of recombination for nuclear and cytoplasmic genes and somatic embryogenesis.In this review,we summarize current progresses on cell wall regeneration of protoplasts.From the separation and purification of plant protoplasts,we discuss the research progress of preparation,culture and plant regeneration.In addition,the factors affecting the regeneration of protoplasts were analyzed.Moreover,the methods of protoplast culture and the application of cell wall regeneration were summarized.Finally,we analyzed the changing progresses during cell wall regeneration and the future application prospect.In summary,this review provides comprehensive view about the development of plant protoplast cell wall regeneration technology.

关 键 词：植物原生质体 原生质体培养 细胞壁再生 遗传改良 

分 类 号：S718.4[农业科学—林学]