白蜡花粉最佳离体萌发培养基筛选  

Screening of optimal germination medium for in vitro Fraxinus

作　　者：刘蓉 吴德军[1] 王因花[1] 任飞[1] 李丽[1] 燕丽萍[1] 周晓锋 LIU Rong;WU Dejun;WANG Yinhua;REN Fei;LI Li;YAN Liping;ZHOU Xiaofeng(Shandong Provincial Academy of Forestry,Shandong Provincial Key Laboratory of Forest Tree Genetic Improvement,Jinan 250014,China;Yantai Institute of China Agricultural University,Yantai 264670,China)

机构地区：[1]山东省林业科学研究院,山东省林木遗传改良重点实验室,山东济南250014 [2]中国农业大学烟台研究院,山东烟台264670

出　　处：《南京林业大学学报（自然科学版）》2023年第3期70-76,共7页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：山东省农业良种工程项目(2019LZGC009);国家重点研发计划项目(2021YFD22003030109)。

摘　　要：【目的】筛选白蜡(Fraxinus sp.)花粉最适离体培养基,能够为后续的授粉受精和育种工作提供一定的参考依据。【方法】通过收集绒毛白蜡‘金箭’(F.velutina‘Jinjian’)和美国红梣‘鲁蜡5号’(F.pennsylvanica‘Lula 5’)的花粉,比较固体培养基和液体培养基上花粉的萌发情况,选用适宜的离体培养法观察培养时间对花粉萌发情况的影响,设计正交试验,筛选最佳离体萌发培养基。【结果】(1)固体培养基上‘金箭’的花粉萌发率均显著高于液体培养基,02和03号固体培养基上‘鲁蜡5号’的花粉萌发率显著高于液体培养基。(2)培养2.5 h左右的花粉适宜进行生活力的观测。(3)正交试验表明:‘金箭’花粉的最适萌发培养基为150 g/L蔗糖+20 mg/L硼酸+30 mg/L氯化钙,‘鲁蜡5号’花粉的最适萌发培养基为150 g/L蔗糖+40 mg/L硼酸+30 mg/L氯化钙。【结论】采用150 g/L蔗糖+20 mg/L硼酸+30 mg/L氯化钙的固体培养基培养2.5 h后适宜统计两种白蜡花粉的萌发情况,但不同种的花粉萌发率与花粉管长度的相关性有差异。【Objective】Screening of the optimal medium in vitro for pollen can provide a valuable reference for subsequent pollination,fertilization and breeding of Fraxinus.【Method】A comparative analysis of pollen germination in Fraxinus velutina cv.Jinjian and F.pennsylvanica cv.Lula 5 was conducted on the solid and liquid media.An appropriate culture method in vitro was selected to observe the influence of culture time on pollen germination and screening for the optimal germination medium in vitro through orthogonal experiments.【Result】The results showed that the pollen germination rate of‘Jinjian’on the solid medium was significantly higher than that on the liquid medium.The pollen germination rate of‘Lula 5’on the solid medium No.02 and 03 was significantly higher than that on the liquid medium.Pollen cultured for approximately 2.5 h was suitable for the observation of viability.The orthogonal test showed that the optimum medium for pollen germination of‘Jinjian’was 150 g/L sucrose+20 mg/L boric acid+30 mg/L calcium chloride.Meanwhile,the optimum medium for pollen germination of‘Lula 5’was 150 g/L sucrose+40 mg/L boric acid+30 mg/L calcium chloride.【Conclusion】Pollen germination of‘Jinjian’and‘Lula 5’was suitable to calculate after 2.5 h of cultivation on the solid medium with 150 g/L sucrose+20 mg/L boric acid+30 mg/L calcium chloride.However,the correlation between the pollen germination rate and pollen tube length of different species was different.

关 键 词：白蜡 花粉 离体培养 培养基 

分 类 号：S722[农业科学—林木遗传育种]