基于成分定量和指纹图谱的化学模式识别法评价胆木不同部位的差异性  

Differences of Nauclea officinalis in different parts based on quantitative analysis of components and fingerprint by chemical pattern recognition

作　　者：彭警 樊箫雨 王迪磊 徐冰[1] 张泽康 肖五庆 杨天姿 李鹏跃[1] 杜守颖[1] PENG Jing;FAN Xiao-yu;WANG Di-lei;XU Bing;ZHANG Ze-kang;XIAO Wu-qing;YANG Tian-zi;LI Peng-yue;DU Shou-ying(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院,北京102488

出　　处：《中草药》2023年第10期3281-3291,共11页Chinese Traditional and Herbal Drugs

摘　　要：目的建立胆木不同部位的UPLC指纹图谱,结合成分定量与化学模式识别,评价胆木不同部位的差异性。方法采用Waters Acquity BEH C18色谱柱(100 mm×2.1 mm,1.7μm);以乙腈(A)-0.1%磷酸水溶液(B)为流动相进行梯度洗脱;检测波长240 nm;体积流量0.3 mL/min;柱温30℃,对胆木不同部位异长春花苷内酰胺的含量进行测定,利用化学模式识别法通过SPSS 20.0对色谱数据进行聚类分析和通过SIMCA 14.1软件对胆木9部位进行主成分分析,并将所有色谱峰化为分类数据对药材不同部位的差异性进行分析。结果对于异长春花苷内酰胺而言,植株地下部位(根皮、去皮根茎)的累积量要高于地上部位的累积量,在地下部位,根皮部位的含量高于去皮根茎部位的含量,地上部位中叶含量最高,其次为茎干部位,最后为枝部位,茎干部位趋势从高到低大致为去皮茎干、带皮茎干、茎皮,枝部位趋势从高到低大致为枝皮、去皮枝茎、带皮小枝。3批次9部位(27份)药材UPLC图谱有9个共有峰,指认了其中獐牙菜苷、绿原酸、马钱苷酸3个色谱峰。当平方欧氏距离为10时,9部位被聚为3类:3批叶聚为一类,3批根皮、3批茎皮、3批枝皮共聚为一类,3批去皮根茎、3批带皮茎干、3批去皮茎干、3批带皮小枝、3批去皮枝茎聚为一类。所有色谱峰化为分类数据后,在皮部位中,根皮能区分于茎皮和枝皮,色谱图中根皮也存在区别于茎皮和枝皮的特征峰;在去皮部位中,去皮根茎可区分于去皮枝茎和去皮茎干,色谱图中去皮根茎也存在区别于去皮枝茎和去皮茎干的特征峰,同时去皮枝茎和去皮茎干可通过色谱图中的特征峰区分开来;在带皮部位中,带皮小枝与带皮茎干可以区分开来。结论胆木皮部位(根皮、枝皮、茎皮)、带皮部位(带皮小枝、带皮茎干)、去皮部位(去皮茎干、去皮枝茎、去皮根茎)、叶部位存在差异,可为胆木的进一步开发利用�Objective To establish UPLC fingerprint of different parts of Nauclea officinalis,and evaluate the quality of different parts of N.officinalis by combining composition quantification and chemical pattern recognition.Methods Waters Acquity BEH C18 column(100 mm×2.1 mm,1.7μm)was used.Gradient elution was carried out with acetonitrile(A)-0.1%phosphoric acid aqueous solution(B)as mobile phase.Detection wavelength:240 nm;Flow rate:0.3 mL/min;The contents of strictosamide in different parts of N.officinalis were determined at 30 o C.Cluster analysis of chromatographic data was performed with SPSS 20.0 by chemical pattern recognition method and principal component analysis was performed with SIMCA 14.1 software for nine parts of N.officinalis.Results The accumulation of strictosamide in the underground part of the plant(root bark,peeled root)was higher than that in the aboveground part,in the underground part,the content of the root bark part was higher than that of the peeled root,but the content of the peeled root was slightly higher in individual batches,in the abo veground part,the leaf part was the highest,followed by the stem part and the last was branch part,the trend of stem part from high to low was about peeled stem,stem and stem bark,but the content of individual batches of stems was higher than that of peeled stems,and the trend of branch part from high to low was branch bark,peeled branch and branchlet.Nine common peaks were identified,three of which were identified as sweroside,chlorogenic acid and loganic acid.When the squared Euclidean distance was 10,nine parts can be well separated,three batches of leaves clustered into one class,three batches of root bark,three batches of stem bark and three batches of branchlets bark clustered into one class,three batches of stem,three batches of branchletslets,three batches of peeled root,three batches of peeled stem and three batches of peeled branchlets clustered into one class.After all chromatographic peaks were classified,the root bark could be distinguished

关 键 词：胆木 指纹图谱 异长春花苷内酰胺 主成分分析 獐牙菜苷 绿原酸 马钱苷酸 正交偏最小二乘判别分析 

分 类 号：R286.2[医药卫生—中药学]