没药化学成分及抗炎活性研究  

Study on chemical constituents and anti-inflammatory activity of Myrrha

作　　者：杨宝 徐莲莲 陈芳有 罗永明[1] YANG Bao;XU Lian-lian;CHEN Fang-you;LUO Yong-ming(School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学药学院,江西南昌330004

出　　处：《中草药》2023年第9期2716-2721,共6页Chinese Traditional and Herbal Drugs

基　　金：江西中医药大学校级科技创新团队发展计划(CXTD22015);江西中医药大学博士科研启动基金项目(2018BSZR003)。

摘　　要：目的研究没药Myrrha二氯甲烷部位化学成分及部分化合物抗炎活性。方法采用多种柱色谱方法和制备液相色谱进行分离纯化,并利用波谱学方法对其结构进行鉴定。通过检测化合物对脂多糖(lipopolysaccharide,LPS)诱导的BV2细胞释放炎症介质NO的抑制能力,来评估所分离化合物的抗炎作用。结果从没药的二氯甲烷部位中分离得到15个化合物,通过波谱数据与文献数据进行对比,分别鉴定为苍术内酯Ⅲ(1)、myrrhterpenoid N(2)、木香烃内酯(3)、莪术二酮(4)、去氢假虎刺酮(5)、eurylosesquiterpenol E(6)、t-杜松醇(7)、10β-hydroxyisodauc-6-en-14-al(8)、oplopanone(9)、去氢木香内酯(10)、4-hydroxy-4,7-dimethyl-α-tetralone(11)、3-hydroxy-6-methylacetophenone(12)、(2E,4E)-6-hydroxy-2,6-dimethylhepta-2,4-dienal(13)、erlangerin D(14)、(-)-dimethylmatairesinol(15)。结论化合物6、14为首次从没药中分离得到,化合物3、4、5、8、10~13、15为首次从没药属植物中分离得到。对化合物1、5、6、7、9、11~15进行了抗炎活性筛选,化合物1、5、6、7、9、11~15均对LPS诱导的BV2细胞炎症反应具有抑制作用。Objective To study the chemical constituents of dichloromethane extract and the anti-inflammatory activities of some compounds from Myrrha.Methods Various column chromatographic methods and preparative liquid chromatography were used to isolate and purify the dichloromethane parts of Myrrha,the structures of the isolated compounds were identified by spectroscopy(MS and NMR)methods.The anti-inflammatory activity of the isolated compounds was assessed by measuring the ability of the compounds to inhibit the release of the inflammatory mediator NO from BV2 cells induced by lipopolysaccharide(LPS).Results Fifteen compounds were isolated from the dichloromethane part of Myrrha and were identified by comparison of spectral data with literature data as atractylenolideⅢ(1),myrrhterpenoid N(2),costunolide(3),curdione(4),dehydrocarissone(5),eurylosesquiterpenol E(6),tcadinol(7),10β-hydroxyisodauc-6-en-14-al(8),oplopanone(9),dehydrocostus lactone(10),4-hydroxy-4,7-dimethyl-α-tetralone(11),3-hydroxy-6-methylacetophenone(12),(2E,4E)-6-hydroxy-2,6-dimethylhepta-2,4-dienal(13),erlangerin D(14),and(-)-dimethylmatairesinol(15).Conclusion Compounds 6 and 14 are isolated from the species for the first time,while compounds 3,4,5,8,10−13,and 15 are isolated from the genus Commiphora for the first time.Compounds 1,5,6,7,9,and 11−15 are screened for anti-inflammatory activity and compounds 1,5,6,7,9 and 11−15 inhibit the lipopolysaccharide-induced inflammatory response in BV2 cells.

关 键 词：没药 倍半萜 抗炎活性 去氢假虎刺酮 木香烃内酯 莪术二酮 去氢木香内酯 

分 类 号：R284.1[医药卫生—中药学]