脉冲标记下甘蔗^(13)C富集及氮磷钾含量变化  

Enrichment of ^(13)C and Changes of N,P,K Contents in Sugarcane Under Pulse Labeling

作　　者：桂意云[1] 李海碧 韦金菊[1] 周会[1] 祝开 赵培方[3] 刘昔辉[1] 区惠平 GUI Yiyun;LI Haibi;WEI Jinju;ZHOU Hui;ZHU Kai;ZHAO Peifang;LIU Xihui;OU Huiping(Sugarcane Research Center,Chinese Academy of Agricultural Sciences/Sugarcane Research Institute,Guangxi Academy of Agricultural Sciences/Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi),Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Sugarcane Genetic Improvement,Nanning,Guangxi 530007;Guangxi South Subtropical Agricultural Science Research Institute,Chongzuo,Guangxi 532400;Sugarcane Research Institute,Yunnan Academy of Agricultural Sciences,Kaiyuan,Yunnan 661699)

机构地区：[1]中国农业科学院甘蔗研究中心/广西农业科学院甘蔗研究所/农业农村部广西甘蔗生物技术与遗传改良重点实验室/广西甘蔗遗传改良重点实验室,广西南宁530007 [2]广西南亚热带农业科学研究所,广西崇左532400 [3]云南省农业科学院甘蔗研究所,云南开远661699

出　　处：《核农学报》2023年第3期586-593,共8页Journal of Nuclear Agricultural Sciences

基　　金：中央引导地方项目(桂科ZY20198005);国家自然科学基金(31860350);广西自然科学基金(2020GXNSFAA297132)。

摘　　要：^(13)C示踪是精准量化植物碳源贡献土壤碳的有效途径。为建立经济适用的甘蔗^(13)C脉冲标记方法,同时获得^(13)C富集蔗叶,本研究采用自制全密闭标记室进行标记试验,供试甘蔗品种为桂糖58号,设置0(CK)、0.32(T1)、0.64(T2)和1.28 g·m-(3 T3)4个Na2^(13)CO3标记浓度,每隔7 d进行6 h的^(13)CO_(2)脉冲标记,共标记6次。于第6次脉冲标记开始及结束后采集标记室内气体,测定CO_(2)浓度和δ^(13)C-CO_(2)。并于第2、第4和第6次标记7 d后破坏性采集植株,测定蔗叶和根系δ^(13)C值及全碳和N、P、K含量,计算蔗叶和根系^(13)C标记效率。结果表明,在密闭标记时间内,外源释放的^(13)CO_(2)95%以上被甘蔗光合作用利用。蔗叶δ^(13)C值和F值随着标记次数和Na2^(13)CO3标记浓度的增加而增加。标记4次和6次后,蔗叶^(13)C平均标记效率分别为25.2%和36.6%。总体来看,每次以0.32 g·m^(-3) Na2^(13)CO3标记6 h,每隔7 d标记1次,标记4次即可获得经济适用的^(13)C富集蔗叶。养分含量结果表明,T1和T2浓度标记的蔗叶N、P、K含量均显著高于或相当于未标记蔗叶。标记4次后,除T2浓度标记的根系P含量外,标记根系的N、P、K含量均较未标记根系降低7.9%~32.9%、0.9%~20.7%和21.9%~47.9%。蔗叶N、K含量与根系N、K含量呈显著或极显著负相关。可见^(13)C脉冲标记影响了甘蔗体内N、P、K的吸收与分配,促进了根系N、K向蔗叶迁移。本研究结果可为甘蔗同位素的高效标记以及标记期间甘蔗的养分管理提供参考依据,并为进一步研究甘蔗秸秆碳在大气-植物-土壤中的周转提供科学材料。^(13)C tracing is an effective way to accurately quantify the contribution of plant carbon sources to soil carbon.In order to establish an economical and applicable ^(13)C pulse labeling method for sugarcane and obtain ^(13)C enriched sugarcane leaves,a self-made fully closed labeling room was used for labeling experiments in this study.The sugarcane variety was GT58.Four Na2^(13)CO3 levels(0,0.32,0.64 and 1.28 g·m^(-3))were conductedand recorded as CK,T1,T2 and T3.^(13)CO_(2) pulse labeling was performed every 7 d for 6 h,a total of 6 times.At the beginning and end of the sixth pulse labeling,the gas in the labeling room was collected,and the CO_(2) concentration andδ^(13)C-CO_(2) were measured.Plants were destructively collected in 7 d after the 2nd,4th and 6th labeling,and theδ^(13)C value,total carbon and contents of N,P and K in sugarcane leaves and roots were measured.The ^(13)C labeling efficiency of sugarcane leaves and roots was further calculated.The results showed that more than 95%of the ^(13)CO_(2) released by the exogenous source was utilized by sugarcane photosynthesis during the sealed marking time.Theδ^(13)C value and F value of cane leaves increased with the increase of labeling times and Na2^(13)CO3 labeling concentration.After 4 and 6 times of labeling,the average labeling efficiency of ^(13)C in sugarcane leaves was 25.2%and 36.6%,respectively.In general,^(13)C enriched sugarcane leaves can be obtain by labeling once every 7 days with 0.32 g·m^(-3) Na2^(13)CO3 for 6 h,and labeling 4 times.The results of nutrient contents showed that the contents of N,P and K in sugarcane leaves marked by T1 and T2 concentrations were significantly higher than or equivalent to those in unlabeled sugarcane leaves.After labeling for 4 times,N,P and K contents in labeled roots decreased by 7.9%~32.9%,0.9%~20.7%and 21.9%~47.9%compared with unlabeled roots except for P content in roots labeled with T2 concentration.The contents of N and K in sugarcane leaves were significantly or extremely significantly negati

关 键 词：碳同位素 甘蔗 ^(13)CO_(2)脉冲标记 氮磷钾含量 

分 类 号：S566.1[农业科学—作物学]