青海省汉族妊娠期高血压疾病与维生素D受体基因FokⅠ多态性的关系  

Relationship between Fok I polymorphism of vitamin-D receptor gene and hypertensive disorder complicating pregnancy in Han nationality of Qinghai province

作　　者：武震 段倩 袁明 王雪妮 陈文静 李玉琴[3] 俄洛吉[3] 李建华 WU Zhen;DUAN Qian;YUAN Ming;WANG Xue-ni;CHEN Wen-jing;LI Yu-qin;E-Luo-Ji;LI Jjian-hua(Department of Human Anatomy,Basic Medicine Science,Medical College of Qinghai University,Xining 810016,China;Department of Ultrasound Medicine,the First Affiliated Hospital of Henan University,He'nan,Kaifeng 475000,China;Department of Obstetrics,Qinghai Provincial People's Hospital,Xining 810007,China)

机构地区：[1]青海大学医学院基础医学部人体解剖学教研室,西宁810016 [2]河南大学第一附属医院超声科,河南开封寸475000 [3]青海省人民医院产科,西宁8810007

出　　处：《解剖学报》2023年第3期313-318,共6页Acta Anatomica Sinica

基　　金：青海省科技厅项目(2019-SF-134);青海省卫生计生系统指导性计划课题(2020-wjzdx-08)。

摘　　要：目的 探讨维生素D受体(VDR)基因单核苷酸多态性(SNP)FokⅠ(rs2228570/rs10735810)位点多态性与青海省汉族妇女妊娠期高血压疾病(HDCP)的相关性。方法 选择青海省汉族HDCP患者137例(HDCP组),正常汉族孕妇146例(对照组),使用聚合酶链式反应-限制性核酸内切酶片段长度多态性(PCR-RFLP)方法检测HDCP组和对照组FokⅠ位点的基因分型并测序验证,使用SPSS 19.0统计学软件检验两组一般临床资料、基因型及等位基因频率分布差异是否具有统计学意义。结果 HDCP组和对照组FokⅠ位点FF、Ff、ff基因型频率分别为51.82%、37.96%、10.22%和34.93%、43.15%和21.92%(χ^(2)=11.099,P<0.05),HDCP组FF基因型(OR=2.004,95%CI=1.243~3.231)频率高于对照组;HDCP组和对照组FokⅠ位点F和f等位基因频率分布差异有统计学意义(χ^(2)=12.454,P<0.001),HDCP组F等位基因(OR=1.253,95%CI=1.105~1.421)频率高于对照组。结论 VDR基因FokⅠ位点多态性与青海省汉族HDCP相关,其中F等位基因可能是HDCP的易感基因,FF基因型可能是HDCP的易感基因型。O]bjective To investigate the relationship between single nucleotide polymorphism(SNP)Fok I(rs2228570/rs10735810)of vitamin D receptor(VDR)gene and hypertensive disorder complicating pregnancy(HDCP)in Han nationality women of Qinghai province.Methods A total of 137 Han nationality HDCP subjects(HDCP group)and 146 Han nationality normal pregnant subjects(control group)were selected from Qinghai province.The Fok I polymorphism typing in HCDP group and control group was analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).The mutation was confirmed by sequencing.SPSS 19.O statistical software was used to test whether there were significant differences between two groups in general clinical data,genotype and allele frequency distribution.Results The frequency of FF Ff ff genotype of Fok I in HDCP group and control group were 51.82%,37.96%,10.22%and 34.93%,43.15%,21.92%respectively(χ^(2)=11.099,P<0.05,the distribution of genotype was different significantly between the two groups),the frequency of FF genotype in HDCP group(OR=2.004,95%CI=1.243-3.231)was higher than that in control group;The frequency distribution of alleles F and f of Fok I was significantly different between HDCP group and control group(χ^(2)=12.454,P<0.001),F allele frequency in HDCP group(OR=1.253,95%CI=1.105-1.421)was higher than that in control group.Conclusion The Fok I polymorphism of VDR gene is significantly correlated with hypertensive disorder complicating pregnancy of Han nationality in Qinghai.The F allele may be the susceptibility gene of HDCP,the FF genotype may be the susceptibility genotype of HDCP.

关 键 词：青海省 妊娠期高血压疾病 维生素D受体 基因多态性 FokⅠ位点 聚合酶链式反应-限制性核酸内切酶片段长度多态性 孕妇 

分 类 号：R3[医药卫生—基础医学]