耐力运动联合间歇性冷刺激促进大鼠白色脂肪组织棕色化  

Endurance exercise combined with intermittent cold stimulation promoting white adipose tissue browning in rats

作　　者：王潇 李梁[1] 崔成立[1] 蔡志平[1] 于畅 张立佳 WANG Xiao;LI Liang;CUI Cheng-li;CAI Zhi-ping;YU Chang;ZHANG Li-jia(School of Basic Medicine and Law,Baotou Medical College,Inner Mongolia Baotou 014040,China;School of Medical Technology and Anesthesia,Baotou Medical College,Inner Mongolia Baotou 014040,China)

机构地区：[1]包头医学院基础医学与法医学院,内蒙古包头014040 [2]包头医学院医学技术与麻醉学院,内蒙古包头014040

出　　处：《解剖学报》2023年第3期348-356,共9页Acta Anatomica Sinica

基　　金：内蒙古自治区自然科学基金(2019MS08199)。

摘　　要：目的探讨耐力运动和间歇性冷刺激对肥胖大鼠棕色脂肪组织活性及白色脂肪组织棕色化的影响及机制。方法52只SD雄性肥胖大鼠分为常温对照组(NC组)、间歇性冷刺激组(IC组)、耐力运动组(EE组)和耐力运动联合间歇性冷刺激组(EI组),每组13只,常温对照组环境温度为24~26℃;运动组采用动物跑台运动,跑速为20 m/min,坡度为0°,每次运动50 min,运动2次,中间间歇20 min;冷刺激组在环境温度为4℃下暴露4 h(低温仓内),每周刺激6 d,持续4周;持续刺激4周后,正电子发射断层显像/X线计算机体层成像(PET/CT)检测大鼠肩胛区棕色脂肪组织(BAT)代谢情况及腹股沟区白色脂肪组织(iWAT)棕色化情况;称体重、量体长、取材,采集血样检测血脂4项;免疫组织化学和Western blotting检测大鼠BAT和iWAT线粒体解耦联蛋白1(UCP1)、血管内皮生长因子A(VEGF-A)、p38 MAPK、过氧化物酶体增殖物激活受体-γ共激活因子-1α(PGC-1α)蛋白表达。结果IC组、EE组、EI组大鼠体重及血清总胆固醇(CHOL)、甘油三酯(TG)、低密度脂蛋白(LDL)显著降低。PET/CT显示,在BAT中,IC组、EE组、EI组最大标准摄取值(SUVmax)显著升高;在iWAT中,EI组SUVmax显著升高。免疫组织化学显示,IC组、EE组、EI组脂肪细胞的数目增多,密度增高,iWAT脂肪细胞中呈现棕色脂肪样细胞。Western blotting显示,在BAT中,IC组、EE组、EI组UCP1蛋白表达水平显著升高;在iWAT中,IC组、EE组、EI组UCP1、PGC-1α蛋白表达水平明显升高;IC组、EI组p38 MAPK蛋白表达水平明显升高;EE组、EI组VEGF-A蛋白表达水平明显降低。结论3种实验方案干预后均能促进肥胖大鼠棕色脂肪组织活化并且促进白色脂肪组织棕色化,以间歇性冷刺激联合耐力运动作用最为显著。Objective To explore the effect and mechanism of endurance exercise and intermittent cold stimulation on brown adipose tissue activity and white adipose tissue browning in obese rats.Methods Totally 52 SD male obese rats were divided into normothermic control group(NC group),intermittent cold stimulation group(IC group),endurance exercise group(EE group)and endurance exercise combined with intermittent cold stimulation group(EI group),13 pieces per group.The ambient temperature of the normothermic control group was 24-26℃;The exercise group used animal running table exercise with a running speed of 20 m/min and a slope of 0°,50 minutes per exercise,exercise 2 times with 20 minutes interval;The cold stimulation group was exposed to an ambient temperature of 4 C for 4 hours(in the cold storage),and stimulated 6 days per week for 4 weeks.After 4 weeks of continuous stimulation,positron emission tomography/computed tomography(PET/CT)was applied to detect the metabolism of interscapular brown adipose tissue(BAT)and browning of inguinal white adipose tissue(iWAT)in rats.Weighting,measuring body length,sampling tissue and collecting blood samples were detected the four lipids.The expression of mitochondrial uncoupling protein 1(UCP1),vascular endothelial growth factor-A(VECF-A),p38 MAPK and peroxisome proliferator-activated receptor coactivator-1(PCC-lα)in interscapular brown adipose tissue and inguinal white adipose tissue of rats were detected by immunohistochemistry and Western blotting.Results The body weight,serum total cholesterol(CHOL),triglyceride(TG)and low density lipoprotein(LDL)of IC,EE and EI groups decreased significantly.PET/CT showed that,in BAT,SUVmax increased significantly in IC,EE and EI groups;In iWAT,maximum standard intake value(SUVmax)increased significantly in EI group.Immunohistochemistry showed:in BAT and iWAT,the number and the density of adipocytes of IC,EE and EI groups increased,and iWAT adipocytes appeared brown adipocytes.Western blotting showed that,in the BAT,the expression level o

关 键 词：耐力运动 冷刺激 白色脂肪组织 棕色脂肪组织 免疫组织化学 免疫印迹法 正电子发射断层显像/X线计算机体层成像 大鼠 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]