电针刺激抑制Nogo-A/NgR通路减轻脑缺血大鼠纹状体白质损伤  

Electroacupuncture stimulation attenuates corpus striatum white matter injury in rats with cerebral ischemia by inhibition of Nogo-A/NgR pathway

作　　者：马同军 董文青 缪化春 吴锋 杨燕萍(译) MA Tongjun;DONG Wenqing;MIAO Huachun;WU Feng;YANG Yanping(Department of Anatomy,Wannan Medical College,Wuhu 241002,China;不详)

机构地区：[1]Department of Anatomy,Wannan Medical College,Wuhu 241002,China [2]不详

出　　处：《Journal of Acupuncture and Tuina Science》2023年第3期173-179,共7页针灸推拿医学（英文版）

基　　金：皖南医学院学术带头人后备人选人才项目,No.校政(2019)81号。

摘　　要：目的:探讨电针(EA)对局灶性脑缺血大鼠纹状体白质损伤的影响及作用机制。方法:无特定病原体Sprague-Dawley大鼠44只,采用随机数字表法分为正常组(normal组,10只)、假手术组(sham组,10只)和造模组(24只)。normal组为空白对照;sham组仅分离血管和迷走神经而不栓塞;造模组采用大脑中动脉栓塞法复制局灶性脑缺血大鼠模型。将模型制备成功的20只大鼠,随机分为模型组(model组)和电针组(EA组),每组10只。Model组大鼠不做进一步处理;EA组大鼠在造模成功后24 h接受EA刺激百会和左侧足三里,每次30 min,每日1次,连续14 d。实验第14天,对各组大鼠进行神经功能缺损评分后处死大鼠,每组5只取缺血灶周围含纹状体的脑组织石蜡包埋。采用神经髓鞘固蓝(LFB)染色检测白质损伤变化;免疫组织化学染色法检测大鼠纹状体中髓鞘碱性蛋白(MBP)、髓鞘相关生长抑制因子(Nogo-A)及其受体(NgR)免疫反应阳性产物的表达。采用蛋白质免疫印迹试验检测缺血灶周围纹状体组织髓鞘相关蛋白MBP、Nogo-A和NgR的表达。结果:与normal组和sham组比较,model组神经功能缺损评分明显增加(P<0.05),呈现出纹状体白质纤维束损伤,表现为纹状体LFB染色的平均光密度值明显降低(P<0.05),MBP表达水平明显降低(P<0.05),Nogo-A和NgR蛋白表达水平明显升高(P<0.05)。与model组相比,EA组神经功能缺损评分明显降低(P<0.05),LFB染色的平均光密度值显著升高(P<0.05),MBP表达水平升高(P<0.05),Nogo-A和NgR蛋白表达下降(P<0.05)。结论:EA能减轻脑缺血诱发的纹状体白质损伤程度,改善神经功能缺损;其作用机制可能与抑制Nogo-A/NgR通路激活有关。Objective:To investigate the effect and the mechanism of electroacupuncture(EA)on corpus striatum white matter injury in rats with focal cerebral ischemia(FCI).Methods:Forty-four specific-pathogen-free Sprague-Dawley rats were divided into a normal group(n=10),a sham-operation group(sham group,n=10),and a modeling group(n=24)using the random number table method.The normal group was a blank control.In the sham group,only the vessels and vagus nerve were isolated without embolization.The FCI rat model in the modeling group was replicated using the middle cerebral artery occlusion embolization method.The 20 successfully modeled rats were randomly divided into a model group and an EA group,with 10 rats in each group.Rats in the model group did not receive further treatment.Rats in the EA group received EA stimulation at Baihui(GV20)and the left Zusanli(ST36)24 h after the successful modeling,30 min each time,once a day for 14 d.On the 14th day of the experiment,rats in each group were scored for neurological deficits and then sacrificed,and brain tissues containing corpus striatum around the ischemic focus were paraffin-embedded from 5 rats in each group.Luxol fast blue(LFB)staining was used to detect damage changes in the white matter.The positive immunoreactive expression of myelin basic protein(MBP),myelin-associated growth inhibitor A(Nogo-A)and its receptor(NgR)in rat corpus striatum tissue was detected by immunohistochemistry staining,and then the protein expression of MBP,Nogo-A,and NgR in the corpus striatum tissue around the ischemic focus was determined by Western blotting.Results:Compared with the normal group and the sham group,the model group had a significantly higher neurological deficit score(P<0.05)and fiber bundle injuries in the corpus striatum white matter,evidenced by a significantly lower mean optical density value of corpus striatum LFB staining(P<0.05),a significantly lower MBP expression level(P<0.05),and significantly higher Nogo-A and NgR protein expression levels(P<0.05).Compared with the

关 键 词：针刺疗法 电针 脑缺血 纹状体 脑白质 NOGO蛋白 NOGO受体 大鼠 

分 类 号：R2-03[医药卫生—中医学]