Greener approach to the comprehensive utilization of algal biomass and oil using novel Clostridial fusants and bio-based solvents  

作　　者：Asma Fiayaz Yaser Dahman 

机构地区：[1]Department of Chemical Engineering,Toronto Metropolitan University,350 Victoria St,Toronto,Ontario M5B 2K3,Canada

出　　处：《Engineering Microbiology》2023年第2期16-23,共8页工程微生物学（英文）

基　　金：Authors would like to acknowledge financial support from Agricul-ture and Agri-Food Canada,the Natural Sciences and Engineering Re-search Council of Canada(NSERC),and the Faculty of Engineering and Architectural Science at Toronto Metropolitan University in Toronto,Canada.

摘　　要：A greener method has been tested to utilize algal biomass as a feedstock to produce bio-oil in addition to acetone,butanol,and ethanol(ABE)products.Various hydrolysis treatments were used prior to fermentation including combination of thermal,chemical,and enzymatic,which resulted in maximum sugar release of 27.78 g/L.Bio-based terpenes was used instead of common toxic chemicals together with Clostridial fustants to produce bio-alcoholic fuels.Protoplast fusion technique were used to produce the novel Clostridia fusants(C.beijernickii+C.thermocellum and C.acetobutylicum+C.thermocellocum).Fused strains were then subjected to UV radiation for strain enhancement.Final fusansts showed clear improvement in thermal stability and resistance to biobutanol toxicity.Fermentation experiments showed maximum biobutanol final production of 7.98 g/L using CbCt versus 7.39 g/L using CaCt.Oil extraction from virgin algae was tested using a green,bio-based approach using ter-penes with ultrasonication and green Bligh and Dyer method,separately.In preliminary study on algal biomass,the combinations of ultrasonication followed by the green Bligh and Dyer have resulted in oil yield of 46.27%(d-limonene)and 39.85%(p-cymene).Oil extraction from an algae sample following fermentation using the combined extraction method resulted in significantly higher oil yield of 65.04%.

关 键 词：Algal biomass BIOBUTANOL Biofuels Simultaneous hydrolysis and fermentation Renewable sources Green products Separate hydrolysis and fermentation Green oil extraction 

分 类 号：Q93[生物学—微生物学]