基于辣椒种间杂交F_(2)群体的遗传连锁图谱构建及主要果实性状QTL定位  

Construction of genetic linkage map and QTL localization of fruit major traits based on interspecific hybrid F_(2)population of pepper

作　　者：袁雷 唐亚萍[2] 张国儒 吉雪花[1] 杨生保[2] YUAN Lei;TANG Yaping;ZHANG Guoru;JI Xuehua;YANG Shengbao(College of Agriculture,Shihezi University/Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization,Shihezi Xinjiang 832003,China;Institute of Horticulture,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)

机构地区：[1]石河子大学农学院/特色果蔬栽培生理与种质资源利用兵团重点实验室,新疆石河子832003 [2]新疆农业科学院园艺作物研究所,乌鲁木齐830091

出　　处：《新疆农业科学》2023年第5期1150-1161,共12页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目(32060676,31860548,32060680);国家现代农业产业技术体系建设专项资金项目(CARS-24-G-29)~~。

摘　　要：【目的】研究辣椒果实主要性状关键基因,为挖掘辣椒果实功能基因和新品种选育提供参考。【方法】以ZL-280(C.chinense)和GB-35(C.annuum)杂交获得的F_(2)代群体为材料,构建辣椒种间遗传图谱,采用复合区间作图法(CIM)果实主要性状进行QTL分析。【结果】构建了一张包含12个连锁群,总图距为1233.87 cM,标记间平均遗传距离为0.59 cM的辣椒高密度种间遗传图谱。共计检测到与色价关联的3个QTL位点,分别为位于第2、4和5条染色体上的cv.2.1、cv.4.1和cv.5.1,贡献率分别为8.58%,10.66%,12.44%;检测到与辣椒红素关联的2个QTL位点,分别为位于第2、5号染色体上的c.2.1和c.5.1,贡献率分别为7.14%和11.40%;检测到与辣椒素关联的3个QTL位点,分别位于第1、2和8号染色体上的cap.1.1、cap.2.1、和cap.8.1,贡献率为13.00%,、18.95%、13.29%;与果实纵径关联的QTL位点有3个,分别为位于第2、4号染色体上的fl.2.1、fl.2.2和fl.4.1,贡献率为6.18%、4.71%和4.01%;与果实横径关联的QTL位点1个,位于第6号染色体上的fd.6.1,贡献率为10.07%;检测到与胎座大小关联的QTL位点2个,分别为位于第2号和第7号染色体上的ps.2.1和ps.7.1,贡献率为4.55%和5.35%;1个与果肉厚关联的QTL位点,为位于第11号染色体上的pt.11.1,贡献率为1.64%;检测到与单果重关联的QTL位点1个,位于第7号染色体上sfw.7.1,贡献率为4.33%。【结论】构建了一张高密度辣椒种间遗传图谱,检测到16个与辣椒果实主要性状关联的QTLs位点,贡献率范围为1.64%~18.95%。【Objective】The mining of key genes for the main traits of pepper fruit is of great significance for promoting the innovation of pepper germplasm and the selection and breeding of new varieties.【Methods】In this study,the F_(2) generation population obtained by the hybridization of ZL-280(C.chinense)and GB-35(C.annuum)was used as the material to construct the interspecific genetic map of pepper,and the main traits of the fruit were analyzed by composite interval mapping(CIM).【Results】A high-density interspecific genetic map of pepper containing 12 linkage groups with a total distance of 1,233.87 cM and an average genetic distance between markers of 0.59 cM was constructed.Through joint data analysis,a total of three QTL sites associated with color valence were detected,namely cv.2.1,cv.4.1 and cv.5.1 located on chromosomes 2nd,4th and 5th,with contribution rates of 8.58%,10.66%and 12.44%,respectively.Two QTL sites associated with capsaicin were detected,c.2.1 and c.5.1 on chromosomes 2 and 5,with contribution rates of 7.14%and 11.40%,respectively.Three QTL sites associated with capsaicin were detected,cap.1.1,cap.2.1 and cap.8.1 located on chromosomes 1,2 and 8,respectively,with a contribution rate of 13.00%,18.95%and 13.29%.There were three QTL sites associated with the longitudinal diameter of the fruit,namely fl.2.1,fl.2.2 and fl.4.1 on chromosomes 2 and 4,with a contribution rate between 6.18%,4.71%and 4.01%.There was 1 QTL site associated with the transverse diameter of the fruit,located on fd.6.1 on chromosome 6,with a contribution rate of 10.07%;Two QTL sites associated with placenta size were detected,respectively,ps.2.1 and ps.7.1 located on chromosomes 2 and 7,with contribution rates of 4.55%and 5.35%;One QTL site associated with thick pulp was pt.11.1 located on chromosome 11,with a contribution rate of 1.64%;One QTL site associated with single fruit reassociation was detected,sfw.7.1 located on chromosome 7,with a contribution rate of 4.33%.【Conclusion】In this study,an interspecific gene

关 键 词：辣椒 果实性状 遗传连锁图谱 QTL定位 

分 类 号：S641.3[农业科学—蔬菜学]