9个欧洲李品种自交不亲和S-RNase 基因的克隆及序列分析  

Cloning and sequence analysis of self-incompatibilityS-RNase gene from 9 European plum cultivars

作　　者：王亚铜 玉米提·玉素甫 耿文娟[2] 章世奎[1] 孙召展 王绍鹏 王尚栋 樊国全[1] WANG Yatong;Yumiti Yusufu;GENG Wenjuan;ZHANG Shikui;SUN Zhaozhan;WANG Shaopeng;WANG Shangdong;FAN Guoquan(Luntai Fruit Tree Germplasm Resource Nursery,Xinjiang Academy of Agricultural Sciences,Luntai Xinjiang 841600,China;College of Horticulture,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业科学院轮台果树资源圃,新疆轮台841600 [2]新疆农业大学园艺学院,乌鲁木齐830052

出　　处：《新疆农业科学》2023年第5期1162-1169,共8页Xinjiang Agricultural Sciences

基　　金：中央财政林草科技推广示范项目横向基金;新疆农业科学院青年科技骨干创新能力培训项目(xjnky-2020026);国家园艺种质新疆名特果树及砧木分库运行服务(NHGRC2022-NH13);新疆特有果树种质资源安全保存及普查收集资源鉴定评价与繁殖编目入圃(19221874)~。

摘　　要：【目的】鉴定9个欧洲李品种自交不亲和S-RNase基因型,为提高生产效率和高效育种提供理论依据。【方法】利用2对蔷薇科通用引物对9个欧洲李品种的叶片基因组DNA进行特异性PCR扩增,片段回收,将克隆测序的结果在GenBank数据库中进行同源性比对并分析序列。【结果】9个欧洲李品种均扩增出2条带,鉴定出9个样品中的8个欧洲李品种的S基因型,其中理查德早生和斯泰勒的S基因型相同为S1S9,法兰西和Silvia的S基因型相同为S1S5,乌孜干1号、总统、Decbrowice和女神的S基因型分别为S1S11、S6SSJ、S1SSJ、SSAS11,塔城酸梅仅鉴定出具有一条S1-RNase等位基因,未能确定出其S基因型。基因频率分析发现S1-RNase基因出现频率最高,S6-RNase和SSA-RNase基因频率最低。欧洲李SSA、S6、S9、SSJ和S5亲缘关系较近,参试李亚科树种的S-RNase基因不能单独聚成一个亚类,而是彼此交错分布在李亚科各个分支中。【结论】欧洲李的S基因型是由2种不同的S等位基因组成,鉴定的9个样品中,有8个欧洲李品种得到完整的S基因型,其中S1-RNase基因出现频率最高,李亚科植物S基因的分化早于各个种形成之前,而S基因种内的进化发生在各自单独的系统中。【Objective】To study cloning and sequence analysis of self-incompatibility S-RNase gene from 9 European plum cultivars【Methods】Leaf genomic DNA of 9 European Prunus varieties were amplified by specific PCR using two pairs of universal primers of Rosaceae,and the fragments were recovered.The cloned sequencing results were compared in GenBank database for homology and sequence analysis.【Result】Two bands were amplified from 9 European plum cultivars and the S genotypes of 8 European plum cultivars in 9 tested samples were identified.The S genotypes of Richard Early and Stanley were S1S9,and French and Silvia were S1S5.The S genotypes of Uzigan 1,President,Decbrowice and Nmpress were S1S11,S6SSJ,S1SSJ and SSAS11,respectively.Only one S1-RNase allele was identified in Tacheng Suanmei,but the S genotype was not identified.Gene frequency analysis showed that the frequency of S1-RNase gene was the highest,and the frequency of S6-RNase and SSA-RNase gene was the lowest.The phylogenetic tree of Rosaceae showed that SSA,S6,S9,SSJ and S5 were closely related to each other.The S-RNase genes of the tested Prunus species could not cluster into a single subclass,but were interleaved with each other in each branch of Prunus.【Conclusion】The S genotype of European plum is composed of two different S alleles.Of the nine samples identified,eight European plum cultivars yield the complete S genotype,in which S1-RNase gene appears the highest frequency.The S gene differentiation of Lyaceae plants is earlier than the formation of each species.Evolution within the S gene species takes place in separate systems.

关 键 词：欧洲李 自交不亲和 S-RNASE基因 克隆 

分 类 号：S662.3[农业科学—果树学]