棉花根际溶磷菌WJP-7发酵培养基优化及防病增产效果  

Inoculation technology and field application of cottonrhizosphere phosphorus-solubilizing P.Taiwanensis WJP-7

作　　者：王宁[1,2] 史应武 牛新湘[2,3] 杨红梅 楚敏 詹发强[1] 包慧芳[1] 杨蓉 龙宣杞[1] 丁荣荣 WANG Ning;SHI Yingwu;NIU Xinxiang;YANG Hongmei;CHU Min;ZHAN Faqiang;BAO Huifang;YANG Rong;LONG Xuanqi;DING Rongrong(Institute of Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences/Xinjiang Special Environmental Microbiology Laboratory,Urumqi 830091,China;Key Laboratory of Northwest Oasis Agricultural Environment,Ministry of Agriculture and Rural Affairs,Urumqi 830091,China;Institute of Soil and Fertilizer and Agricultural Water Conservation,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Seventh Division Agricultural Research Institute,Xinjiang Production and Construction Corps,Kuitun Xinjiang 832000,China)

机构地区：[1]新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐830091 [2]农业农村部西北绿洲农业环境重点实验室,乌鲁木齐830091 [3]新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐830091 [4]新疆生产建设兵团第七师农业科学研究所,新疆奎屯833200

出　　处：《新疆农业科学》2023年第5期1263-1270,共8页Xinjiang Agricultural Sciences

基　　金：新疆生产建设兵团第七师胡杨河市财政科技计划项目(2020C14);国家自然科学基金地区科学基金项目(31860024);新疆农业科学院青年科技骨干创新能力培养项目(xjnkq-2019014);新疆维吾尔自治区科技计划重大专项(2020A01002);自治区重点研发项目(2021B02004)~~。

摘　　要：【目的】研究棉花根际溶磷菌WJP-7发酵培养基优化及防病增产效果。【方法】采用实验室筛选的高效溶磷菌株P.taiwanensis WJP-7为出发菌株,在优化的假单胞菌发酵培养基上增殖培养。【结果】利用正交试验优化摇瓶发酵培养基,培养基配方为蛋白胨20 g/L,MgCl_(2)15 g/L,K_(2)SO_(4)1.4 g/L。使活菌数达到3.6×10^(9)CFU/mL。摇瓶增菌发酵最优培养基发酵验证试验结果最高活菌数为3.60×10^(9)CFU/mL。菌株WJP-7水剂处理对花期黄萎病防治效果为44.57%,絮期防治效果为50.53%。施用WJP-7水剂对棉田土壤除全氮含量增加不显著,对其它养分和pH值均有显著影响(P<0.05)。菌剂增产率为14.35%和19.51%,【结论】优化了台湾假单胞菌WJP-7摇瓶发酵培养基,菌株WJP-7水剂处理对棉花黄萎病防治效果为50.53%,对棉田土壤除全氮含量不显著外,对其它养分和pH值均显著影响(P<0.05),具有明显的增产效果。【Objective】Inoculation technology and field application of cotton rhizosphere phosphorus-solubilizing P.Taiwanensis WJP-7.【Method】In this paper,the self-selected high-efficiency phosphorus-dissolving strain P.taiwanensis WJP-7 was used as the starting strain,and it was propagated and cultured in the optimized Pseudomonas fermentation medium.【Results】The results showed that the shake flask fermentation medium was optimized by orthogonal experiment,and the medium formula was determined as:peptone 20 g/L,magnesium chloride 15 g/L,potassium sulfate 1.4 g/L.The number of viable cells reached 3.6×10^(9) CFU/mL.The maximum viable bacteria count of the optimal medium for enrichment fermentation in shake flask was 3.60×10^(9) CFU/mL.The control effect of strain WJP-7 water treatment on Verticillium wilt at flowering stage was 44.57%,and the control effect at flocculation stage was 50.53%.The application of microbial inoculants on cotton field soils had no significant increase in total nitrogen content(P>0.05),but significant effects on other nutrients and pH values(P<0.05).The yield increase rates were 14.35%and 19.51%.【Conclusion】The shake flask fermentation medium of Pseudomonas Taiwan WJP-7 was optimized by orthogonal experiment.Except for the total nitrogen content in cotton field soil,it has a significant effect on other nutrients and pH value(P<0.05),with a significant yield increase effect.

关 键 词：棉花 溶磷菌 增菌工艺 田间应用 

分 类 号：S512[农业科学—作物学] S188