基于全基因组重测序分析新疆细毛羊遗传多样性  

Genetic diversity analysis of xinjiang sheep withfine wool based on whole-genome Re-sequencing

作　　者：陈开旭 郭翠洁 杨帆[1] 任斐儿 李晓斌[1] 刘武军[1] CHEN Kaixu;GUO Cuijie;YANG Fan;REN Feier;LI Xiaobin;LIU Wujun(College of Animal Science,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052

出　　处：《新疆农业科学》2023年第5期1292-1300,共9页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区高校科研计划项目“基于简化基因组的新疆地方绵羊品种群体遗传学分析”(XJEDU2020Y014);中国博士后科学基金“新疆绵羊线粒体DNA遗传多样性研究”(2017M613307XB)~~。

摘　　要：【目的】基于全基因组重测序研究新疆细毛羊遗传多样性。【方法】利用新疆细毛羊的全基因组重测序数据和NCBI下载获得的策勒黑羊、巴音布鲁克羊、阿勒泰羊全基因组重测序数据,检测不同绵羊品种的核苷酸多态性和单倍型多态性,评估其遗传多样性现状,计算群体分化指数、观望/观测杂合度,分析遗传多样性,评价不同绵羊品种现有的遗传变异水平。【结果】全基因组重测序获得了97647435个高质量的常染色单核苷酸多态性(SNP)位点和15886270个插入或缺失(Indel);新疆细毛羊的遗传多样性水平显著低于阿勒泰羊,低于巴音布鲁克羊,略高于策勒黑羊;4个绵羊群体遗传多样性顺序:阿勒泰羊>巴音布鲁克羊>策勒黑羊>新疆细毛羊。【结论】选取的4个绵羊群体中,新疆细毛羊的遗传多样性水平相对较低。【Objective】To study the genetic diversity of Xinjiang sheep with fine wool by whole-genome resequencing.【Methods】Whole-genome resequencing data of Xinjiang sheep with fine wool and whole-genome resequencing data of Bayinbruck,Altay,and Qira black sheep downloaded from NCBI were used to assess the existing genetic diversity status of different sheep breeds by detecting nucleotide polymorphism,calculating population differentiation index,watching/observing heterozygosity,and conducting genetic diversity analysis.【Results】97647435 high-quality autosomal single nucleotide polymorphism(SNP)loci and 15886270 insertions or deletions(Indel)were obtained by whole-genome resequencing.The genetic diversity level of Xinjiang fine-wool sheep was significantly lower than that of Altay sheep and that of Bayinbruck sheep and slightly higher than that of Qira black sheep.The order of genetic diversity of 4 sheep populations was:Altay sheep>Bayinbruck sheep>Qira black sheep>Xinjiang fine-wool sheep.【Conclusion】The genetic diversity of Xinjiang fine-wool sheep is relatively low in the 4 sheep populations chosen in this study.

关 键 词：新疆细毛羊 遗传多样性 全基因组重测序 

分 类 号：S813.1[农业科学—畜牧学]