高速逆流色谱法提取分离天然产物中生物碱的应用  

Application of high-speed counter current chromatography in extraction and separation of alkaloids in natural products

作　　者：郭好好 王晶 陈圆 辛秀兰[2] 刘玉峰[1] GUO Hao-hao;WANG Jing;CHEN Yuan;XIN Xiu-lan;LIU Yu-feng(School of Pharmaceutical Sciences,Liaoning University,Shenyang 110036,China;College of Bioengineering,Bejing Polytechnic,Beijing 100176,China)

机构地区：[1]辽宁大学药学院,辽宁沈阳110036 [2]北京电子科技职业学院生物工程学院,北京100176

出　　处：《中国中药杂志》2023年第8期1989-1999,共11页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81403177);辽宁省高等学校创新人才项目(LR2018047);辽宁省自然科学基金项目(2021-MS-150);天然产物国家标准样品研制及产品开发技术研究项目(SG030801)。

摘　　要：生物碱类化合物在植物中广泛存在,具有一系列药理活性,并已被广泛用于治疗各种疾病。由于生物碱通常存在于多组分混合物中且含量较低,较难通过传统方法进行提取分离。高速逆流色谱法是一种不存在固态支撑相的液-液色谱技术,具有进样量大、成本低、不存在不可逆吸附等优势。相比于传统的生物碱提取分离方法,高速逆流色谱法可选的溶剂体系和洗脱模式可以保证一次性分离多种不同极性生物碱,可实现生物碱类化合物的高回收及大量制备。该研究通过借鉴相关文献,讨论了高速逆流色谱(HSCCC)相比于传统分离手段的优缺点,并对近年来采用高速逆流色谱法分离生物碱类化合物所用的溶剂系统和洗脱模式进行了总结,旨在为生物碱类化合物的高速逆流色谱分离提供一些参考。Alkaloids, widespread in plants, have a series of pharmacological activities and have been widely used to treat various diseases. Because alkaloids are usually presented in multicomponent mixtures and are deeply low in content, they are very difficult to extract and separate by traditional methods. High-speed counter current chromatography(HSCCC) is a kind of liquid-liquid chromatography without solid support phase, which has the advantages of large injection volume, low cost, and no irreversible adsorption. Compared with the traditional methods of extraction and separation of alkaloids, HSCCC can ensure the separation of many different alkaloids at one time, with a high recovery and large amount. In this paper, the advantages and disadvantages of HSCCC compared with traditional separation methods were discussed and the solvent system and elution mode of HSCCC used to separate alkaloids in recent years were summarized by referring to the relevant literature to provide some references for the separation of alkaloids by HSCCC.

关 键 词：高速逆流色谱 生物碱 提取分离 溶剂系统 洗脱模式 

分 类 号：R284[医药卫生—中药学] O652.63[医药卫生—中医学]