在线二维液相色谱技术用于天然产物活性成分筛选中的研究进展  

Advances in online two-dimensional liquid chromatography for natural product screening

作　　者：袁嘉明 赖亮 汪锦才 张婷婷 江正瑾 YUAN Jia-ming;LAI Liang;WANG Jin-cai;ZHANG Ting-ting;JIANG Zheng-jin(Institute of Pharmaceutical Analysis,College of Pharmacy,Jinan University,Guangzhou 511436,China)

机构地区：[1]暨南大学药学院,药物分析研究中心,广东广州511436

出　　处：《药学学报》2023年第5期1128-1137,共10页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(82073806);广东省自然科学基金资助项目(2021A0505020014);广东省基础与应用基础研究基金自然科学基金资助项目(2020A1515110867)。

摘　　要：高效快速地分离和筛选复杂体系中的活性成分是从天然产物中发现先导化合物的关键。近年来,在线二维液相色谱技术因具有分辨率高、自动化程度高以及集成方式灵活等优势在天然产物活性成分筛选研究中备受青睐。本文简要综述了在线二维液相色谱技术的特点及其在天然产物活性成分筛选领域中的应用研究进展,主要从柱前、柱上以及柱后三大筛选模式的不同集成方式展开叙述,并指出其不足,以期为后续应用研究提供科学参考,促进该技术在活性成分筛选领域的快速发展。As a treasure resource of novel drug lead compounds,how to rapidly and high-efficiently screen and isolate active components from natural products is critical.Thanks to its high resolution,high automation and flexible integration,online two-dimensional liquid chromatography has great potential for screening active ingredients from complex matrices by integrating a highly specific bio-recognition process into a two-dimensional liquid chromatography system before,on or after the column separation.This review comprehensively summarized recent developments,applications and shortcomings of online two-dimensional liquid chromatography for natural product screening from different integration modes,including pre-column,on-column and post-column screening methods.

关 键 词：二维液相色谱 在线 天然产物 活性成分筛选 

分 类 号：R917[医药卫生—药物分析学]