Dual-emission carbonized polymer dots for ratiometric sensing and imaging of L-lysine and pH in live cell and zebrafish  

作　　者：Xiaoxiao Hu Hongjing Wu Qiang Zhang Feng Gao 

机构地区：[1]Laboratory of Functionalized Molecular Solids,Ministry of Education,Anhui Key Laboratory of Chemo/Biosensing,College of Chemistry and Materials Science,Anhui Normal University,Wuhu 241002,China

出　　处：《Chinese Chemical Letters》2023年第5期374-380,共7页中国化学快报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Nos.21874001,21575004);the Foundation for Innovation Team of Bioanalytical Chemistry of Anhui Province。

摘　　要：It is highly desired to accurately and selectively detect and image intracellular L-lysine and pH in biological systems because they could act as the biomarkers in certain abnormal conditions and may give us a warning of the occurrence of diseases.It has been attracted more focuses to design new ratiometric fluorescent probe for monitoring L-lysine and pH to improve detection accuracy.Carbonized polymer dots(CPDs),which possess carbon/polymer hybrid structure rather than pure carbon structure and constitute of a carbon core and large amounts of functional groups/polymer chains on the surface,rise up as a new type of fluorescent nanomaterials and especially display many advantages for bioanalysis.In this study,o-phenylenediamine(o-PD)and poly(styrene-co-maleic anhydride)(PSMA)are used as the precursors to synthesize the desired CPDs through one-step hydrothermal amide method.The prepared CPDs display two well-resolved fluorescence emission bands,i.e.,a very weak emission centered at 470 nm in blue region and a strong emission centered at 558 nm in yellow region.It is found that the two emissions are both responsive to L-lysine based on the surface passivation mechanism,whereas,only the yellow emission is responsive to pH due to the protonation/deprotonation process of the amino groups.Based on the different responsive behaviors,ratiometric detection and imaging of L-lysine and pH are achieved.The prepared ratiometric CPDs probe is successfully applied for L-lysine and pH sensing and imaging at two emission channels in live cell and zebrafish with satisfactory results.

关 键 词：Carbonized polymer dots(CPDs) Ratiometric fluorescent probe L-LYSINE pH Dual-emission 

分 类 号：R318.08[医药卫生—生物医学工程] TB383.1[医药卫生—基础医学] O657.3[一般工业技术—材料科学与工程]