血清经56℃加热30 min的灭活方式对生化项目检测结果的影响  

Effect of inactivation of serum heated at 56℃for 30 min on biochemical test results

作　　者：李欢 尹笑笑 纵如原 杨伏猛[1] 刘倩[1] Li Huan;Yin Xiaoxiao;Zong Ruyuan;Yang Fumeng;Liu Qian(Department of Laboratory,The Second People's Hospital of Lianyungang,Lianyungang Jiangsu 222006,China;Department of Laboratory,Neijiang Traditional Chinese Medicine Hospital,Neijiang Sichuan 641199,China)

机构地区：[1]连云港市第二人民医院检验科,江苏连云港222006 [2]内江市中医院检验科,四川内江641199

出　　处：《中华临床实验室管理电子杂志》2022年第4期204-209,共6页Chinese Journal of Clinical Laboratory Management(Electronic Edition）

基　　金：江苏省卫健委医学科研面上项目(M2020079);蚌埠医学院自然科学基金重点项目(2020byzd342)。

摘　　要：目的基于新型冠状病毒(SARS-CoV-2)的高传染性和致病性,探讨血清经56℃加热30 min对常规生化指标检测结果是否有影响,旨在为实验室通过加热灭活标本开展常规生化指标的检测提供重要参考。方法随机收集2021年1月至连云港市第二人民医院体检中心的体检健康人群50例作为研究对象,应用贝克曼AU5800全自动生化分析仪分别测定灭活前与灭活后的血清常规生化项目,并比较56℃加热30 min的灭活方式对常规生化项目的测定有无影响。结果灭活前与灭活后的血清总蛋白(TP)、尿素(UREA)、肌酐(Cr)、尿酸(UA)、钾(K)、钙(Ca)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)的检测结果差异无统计学意义(P>0.05)。且Bland-Altman一致性分析显示:血清总胆红素(TBIL)、钠(Na)、氯(Cl)、磷(P)、二氧化碳结合力(CO_(2)-CP)、葡萄糖(GLU)、糖化血清蛋白(GSP)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、载脂蛋白A(Apo-A)及载脂蛋白B(Apo-B)在标本灭活前后的差异具有临床可接受的一致性;而血清直接胆红素(DBIL)、白蛋白(ALB)、β_(2)微球蛋白(β_(2)-MG)及同型半胱氨酸(HCY)项目则无临床可接受的一致性。结论实验室基于56℃加热30 min处理血清标本时,需对有影响的生化项目采取必要的校正措施,以期为临床提供精准的检测结果。Objective According to the high infectivity and pathogenicity of SARS-CoV-2,this study investigated whether the serum heated at 56℃for 30 minutes would affect the results of biochemical indicators,so as to provide an important reference for the laboratory to carry out the detection of routine biochemical indicators by heating inactivated specimens.Methods A total of 50 healthy people with physical examination in our hospital from January 2021 were enrolled in this study.The Beckman AU5800 automatic biochemical analyzer was used to determine the serum routine biochemical indicators before and after inactivation,and compared whether the inactivation method of heating at 56℃for 30 min had any effect on the measured results.Results There were no significant differences in the results of serum total protein(TP),urea(UREA),creatinine(Cr),uric acid(UA),potassium(K),calcium(Ca),triglyceride(TG)and low density lipoprotein cholesterol(LDL-C)before and after inactivation(P>0.05).The Bland Altman consistency analysis showed that the differences of serum total bilirubin(TBIL),sodium(Na),chlorine(Cl),phosphorus(P),carbon dioxide binding capacity(CO_(2)-CP),glucose(GLU),glycosylated serum protein(GSP),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),apolipoprotein a(Apo-A)and apolipoprotein B(Apo-B)before and after inactivation were clinically acceptable.However,serum direct bilirubin(DBIL),albumin(ALB),β_(2)-microglobulin(β_(2)-MG),homocysteine(HCY)did not have clinically acceptable consistency.Conclusions When laboratory process the specimen using the method of heating at 56°C for 30 minutes,it is necessary to take corrective measures for the biochemical indicators with significant influence,in order to provide accurate results.

关 键 词：新型冠状病毒 加热灭活 常规生化 

分 类 号：R446.1[医药卫生—诊断学]