不同基原蔓荆子的HPLC指纹图谱及主要成分含量比较  

Comparison of HPLC fingerprints and determination of main components of Viticis Fructus from different species

作　　者：杨晓芸 高佩云 陈晓旭 王丽霞 蒋彤 吴桐 陈迎迎 岳春雨 吴宏伟[1] 唐力英[1] 王祝举[1] YANG Xiao-yun;GAO Pei-yun;CHEN Xiao-xu;WANG Li-xia;JIANG Tong;WU Tong;CHEN Ying-ying;YUE Chun-yu;WU Hong-wei;TANG Li-ying;WANG Zhu-ju(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]河南中医药大学药学院,河南郑州450046

出　　处：《中国中药杂志》2023年第9期2471-2479,共9页China Journal of Chinese Materia Medica

基　　金：山东省重点研发计划项目(2021SFGC1202)。

摘　　要：为综合评价蔓荆子质量,建立蔓荆子的HPLC指纹图谱,通过相似度评价及多元统计分析对不同基原的24批次蔓荆子药材的质量进行评价。在此基础上建立HPLC含量测定方法,比较主要成分蔓荆子黄素、穗花牡荆苷、异荭草素、对羟基苯甲酸的含量差异。色谱条件为Waters Symmetry C_(18)色谱柱,流动相为乙腈(A)-0.05%磷酸水(B)溶液,梯度洗脱,检测波长258 nm,流速1 mL·min^(-1),进样量10μL,柱温30℃。24批蔓荆子指纹图谱共标示了21个共有峰,指认出9个成分。综合24批蔓荆子相关色谱数据进行相似度分析,单叶蔓荆除DYMJ-16外,其相似度均≥0.900,蔓荆的相似度均≤0.864。另外,分别将单叶蔓荆和蔓荆进行相似度分析,结果显示,16批单叶蔓荆的相似度在0.894~0.997,8批蔓荆的相似度在0.990~0.997。表明单叶蔓荆和蔓荆的指纹图谱相似度有所差异,而相同基原间其相似度良好。3种多元统计分析结果均将单叶蔓荆和蔓荆分为2类,并且PLS-DA的VIP分析结果表明,蔓荆子黄素、穗花牡荆苷2个成分对区分单叶蔓荆和蔓荆的贡献最大。含量测定结果显示,不同基原的蔓荆子中异荭草素和对羟基苯甲酸含量无明显差异,但蔓荆子黄素和穗花牡荆苷在不同基原间含量差异显著(P<0.01),单叶蔓荆中蔓荆子黄素含量较高,而穗花牡荆苷在蔓荆中的含量更高。该研究表明不同基原的蔓荆子的指纹图谱相似度和成分含量均存在差异,可为蔓荆子的质量研究以及临床应用提供参考。In order to comprehensively evaluate the quality of Viticis Fructus,this study established HPLC fingerprints and evaluated the quality of 24 batches of Viticis Fructus samples from different species by similarity evaluation and multivariate statistical analysis(PCA,HCA,PLS-DA).On this basis,an HPLC method was established to compare the content differences of the main components,including casticin,agnuside,homoorientin,and p-hydroxybenzoic acid.The analysis was performed on the chromatographic column(Waters Symmetry C_(18))with a gradient mobile phase of acetonitrile(A)-0.05%phosphoric acid solution(B)at the flow rate of 1 mL·min^(-1) and detection wavelength of 258 nm.The column temperature was 30℃and the injection volume was 10μL.The HPLC fingerprint of 24 batches of Viticis Fructus samples was established with 21 common peaks,and nine peaks were identified.Similarity analysis was carried out based on chromatographic data of 24 batches of chromatographic data of Viticis Fructus,and the results showed that except for DYMJ-16,the similarity of Vitex trifolia var.simplicifolia was≥0.900,while that of V.trifolia was≤0.864.In addition,the similarity analysis of two different species showed that the similarity of 16 batches of V.trifolia var.simplicifolia was 0.894-0.997 and that of the eight batches of V.trifolia was between 0.990 and 0.997.The results showed that the similarity of fingerprints of these two species was different,but the similarity between the same species was good.The results of the three multivariate statistical analyses were consistent,which could distinguish the two different species.The VIP analysis results of PLS-DA showed that casticin and agnuside contributed the most to the distinction.The content determination results showed that there was no significant difference in the content of homoorientin and p-hydroxybenzoic acid in Viticis Fructus from different species,but the content of casticin and agnuside was significantly different in different species(P<0.01).The content of casticin w

关 键 词：蔓荆 单叶蔓荆 高效液相色谱法 指纹图谱 含量测定 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]