水稻光温敏不育系gy157S的叶色表型鉴定与候选基因分析  

Leaf Color Phenotype Identification and Candidate Gene Analysis of Photo-Thermo-Sensitive Male Sterile Line gy157S in Rice

作　　者：谢英 陈能刚 张宏伟 郝留根 王珍珍 易崇粉 韩丽珍 杨占烈 XIE Ying;CHEN Nenggang;ZHANG Hongwei;HAO Liugen;WANG Zhenzhen;YI Chongfen;HAN Lizhen;YANG Zhanlie(Key laboratory of Plant Resource Conservation and Germplasm Inovation in Mountainous Region(Ministry of Education),College of Life Sciences/lnstitute of Agrobioengineering,Guizhou University Guiyang,Guizhou 550025;Institute of Crop Gemplasm Resources,Guizhou Acadey of Agricultural Sciences,Guiyang,Guizhou 550006;Rice Institute,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550006)

机构地区：[1]贵州大学生命科学学院/农业生物工程研究院,山地植物资源保护与种质创新教育部重点实验室,贵州贵阳550025 [2]贵州省农作物品种资源研究所,贵州贵阳550006 [3]贵州省水稻研究所,贵州贵阳550006

出　　处：《核农学报》2023年第4期661-671,共11页Journal of Nuclear Agricultural Sciences

基　　金：贵州省科学技术基金(黔科合基础[2020]1Y100);国家水稻产业技术体系(CARS-01)。

摘　　要：为探明光温敏不育系gy157S黄绿叶基因gy157S(t)调控水稻叶色的分子机制及应用潜力,本研究对水稻光温敏核不育系gy157S进行不同温度处理,观察其表型、叶绿素含量以及叶肉细胞的变化情况,并对其进行遗传分析、基因定位和候选基因分析。表型鉴定结果发现,与对照C815S相比,20℃条件下gy157S叶片呈现黄绿色表型,光合色素含量极显著降低,叶绿体发育缺陷严重;30℃条件下gy157S叶片呈现淡绿色表型,光合色素含量仍然显著降低,仍有部分叶绿体发育畸形。遗传分析结果表明,gy157S的黄绿叶表型受一对隐性核基因控制;群体分离分析(BSA)和连锁分析结果将该基因定位于第3号染色体RM15678和RM15824之间,物理距离为2.4Mb;候选基因功能分析、测序和实时荧光定量PCR(qRT-PCR)结果显示,编码铁氧还蛋白OsFdC2的基因OsR498G0306815500.01在第7外显子上存在单核苷酸多态性(SNP)变异,导致编码氨基酸发生改变,可能是引起黄绿叶突变表型的位点。本研究结果为阐明gy157S(t)基因对叶绿体发育和叶绿素合成的分子调控机制奠定了基础。To explore the molecular mechanism and utilization potentiality of the yellow green leaf gene gy157S(t)on regulating rice leaf color.Phenotypic identification,genetic analysis,gene mapping and candidate gene analysis of the rice yellow-green leaf photo-thermo-sensitive male sterile line gy157S at different temperatures were carried out.The results suggested that gy157S displayed yellow-green leaves and pale green leaves when grown at 20℃and 30℃,respectively,with significantly decreased photosynthetic pigment when compared with that of the control C815S,and defective chloroplasts.Genetic analysis showed that the yellow-green leaf phenotype of gy157S was controlled by a recessive nuclear gene.BSA analysis and linkage analysis showed that the gene was located between RM15678 and RM15824 on chromosome 3,with a physical distance of 2.4Mb.Functional analysis,sequencing and qRT-PCR results of candidate genes showed that there was a SNP mutation in exon 7 of OSR498G030681550001 encoding ferredoxin OsFdC2 in gy157S,resulting in the change of amino acid,which may be responsible for the yellow green leaf phenotype.These results lay a foundation for elucidating the molecular regulation mechanism of gy157S(t)gene on chloroplast development and chlorophyll synthesis.

关 键 词：水稻 黄绿叶突变体 铁氧还蛋白 基因表达分析 gy157S 

分 类 号：S511[农业科学—作物学]