防御假单胞菌H78中crp基因对抗生素合成的调控作用  

Regulation of the crp gene on antibiotic biosynthesis in Pseudomonas protegens H78

作　　者：聂晨曦 向涛 张雪洪[1] 黄显清[1] NIE Chenxi;XIANG Tao;ZHANG Xuehong;HUANG Xianqing(State Key Laboratory of Microbial Metabolism,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学生命科学技术学院微生物代谢国家重点实验室,上海200240

出　　处：《生物学杂志》2023年第3期11-15,共5页Journal of Biology

基　　金：上海市自然科学基金项目(19ZR1427600);国家自然科学基金项目(31470196)。

摘　　要：crp基因(编码cAMP受体蛋白)作为全局调控因子广泛存在于各种细菌中,在不同菌株中表现不同的调控功能。防御假单胞菌(Pseudomonas protegens)H78能产生硝吡咯菌素(Prn)、2,4-二乙酰基间苯三酚(2,4-DAPG)和藤黄绿菌素(Plt)等多种广谱抗生素。为探究P.protegens H78中crp基因对抗生素Prn、2,4-DAPG和Plt合成的调控作用,利用基因无痕敲除、HPLC分析、lacZ报告基因分析等技术,分析crp基因对抗生素Prn、2,4-DAPG和Plt合成及基因表达的影响。结果表明:H78菌株中crp基因的敲除导致Prn合成操纵子prnABCD表达显著下降;Plt合成及操纵子表达显著上升;2,4-DAPG合成及操纵子表达小幅下降。因此,P.protegens H78中crp基因对抗生素Prn合成存在显著的正调控作用,而对Plt合成存在明显的负调控作用,对2,4-DAPG合成存在一定的正调控作用。The crp gene(encoding the cAMP receptor protein),as a global regulatory gene,widely exists in various bacteria and exhibits different regulatory functions in different strains.P.protegens H78 is capable of producing multiple broad-spectrum antibiotics such as pyrrolnitrin(Prn),2,4-diacetylphloroglucinol(2,4-DAPG),and pyoluteorin(Plt).In order to investigate the global regulatory effect of the crp gene on the synthesis of antibiotics including Prn,2,4-DAPG and Pltin P.protegens H78,the influences of crp gene on the synthesis and gene expression of these three antibiotics were analyzed by gene knockout,HPLC analysis,and lacZ reporter gene analysis.The results suggested that the knockout of crp gene in H78 strain significantly inhibited Prn synthesis operon(prnABCD)expression,significantly upregulated Plt production and its operon expression,and slightly down regulated 2,4-DAPG production and its operon expression.Therefore,the crp gene exhibited a significant positive regulation on Prn biosynthesis,a significant negative regulation on Plt biosynythesis,and a small positive regulation on 2,4-DAPGbiosynthesis in P.protegens H78.

关 键 词：防御假单胞菌H78 crp基因 抗生素合成调控 硝吡咯菌素 2 4-二乙酰基间苯三酚 藤黄绿菌素 

分 类 号：Q78[生物学—分子生物学] Q939.92