一株BDE-209降解菌的筛选及其降解特性  

Screening and degradation characteristics of a BDE-209 degrading bacterium

作　　者：范罗圣 吴涓[1] 胡丁璠 FAN Luosheng;WU Juan;HU Dingfan(School of Resources and Environmental Engineering,Anhui University,Hefei 230601,China)

机构地区：[1]安徽大学资源与环境工程学院,合肥230601

出　　处：《生物学杂志》2023年第3期41-47,共7页Journal of Biology

基　　金：国家自然科学基金项目(31070100)。

摘　　要：为探究细菌对十溴联苯醚(BDE-209)的生物降解特性,从活性污泥中分离出一株有效降解BDE-209的好氧细菌——硝基还原假单胞菌(Pseudomonas nitroreducens)。研究该菌降解BDE-209的最适外加碳源,探索P.nitroreducens细胞表面特性在不同BDE-209初始质量浓度下的变化及降解动力学特征。结果表明,P.nitroreducens能够以BDE-209为唯一碳源生长。葡萄糖对BDE-209生物降解的促进作用最为显著,当葡萄糖质量浓度为250 mg/L时,对10 mg/L BDE-209的降解率可达76.2%。BDE-209的降解动力学符合准一级动力学方程,半衰期短、降解速率较快,且P.nitroreducens对较高质量浓度的BDE-209具有良好的耐受性。高质量浓度的Cu^(2+)(≥30 mg/L)会抑制BDE-209的降解和菌株的生长。P.nitroreducens的高细胞表面疏水性使BDE-209更容易进入细胞内部,细胞膜通透性的增加是受到BDE-209的应激作用所致。微生物降解是一种将BDE-209从污染环境中清除的有效修复技术,从环境中筛选出高效降解菌为BDE-209的生物降解提供了更多可能性。To investigate the degradation characteristics of decabromodiphenyl ether(BDE-209)by bacteria,an effective aerobic bacterium for degrading of BDE-209 was isolated from activated sludge and identified as Pseudomonas nitroreducens.The optimal additional carbon sources for the biodegradation of BDE-209 were investigated,and the changes of cell surface characteristics and degradation kinetics at different initial mass concentrations of BDE-209 by P.nitroreducens were explored.The experimental results showed that P.nitroreducens was able to grow with BDE-209 as the sole carbon source.Glucose played a significant role in promoting the biodegradation of BDE-209,and the degradation rate of 10 mg/L BDE-209 could reach 76.2% when the glucose mass concentration was 250 mg/L.The degradation kinetics of BDE-209 was in accordance with pseudo first order reaction kinetic equation with short half-life and fast degradation rate.Moreover,P.nitroreducens had a good tolerance to higher mass concentration of BDE-209.High mass concentration of Cu^(2+)(≥30 mg/L)inhibited the degradation of BDE-209 and the growth of P.nitroreducens.The high cell surface hydrophobicity of P.nitroreducens made BDE-209 enter the cells more easily.The increase in cell membrane permeability was caused by the stressful effects of BDE-209.The microbial degradation is an effective remediation strategy to remove BDE-209 from the polluted environment,and the effective screening of degrading bacteria from the environment provided more possibilities for BDE-209 biodegradation.

关 键 词：BDE-209 生物降解 降解动力学 Cu^(2+) 细胞表面特性 

分 类 号：Q935[生物学—微生物学]