多菌种固态发酵玉米皮制备菌体蛋白饲料  

Cell protein feed was prepared by multi-strain solid state fermentation of corn husk

作　　者：高俊杰 燕亚平 李永丽 刘占英[1,2,3] GAO Junjie;YAN Yaping;LI Yongli;LIU Zhanying(Center for Energy Conservation and Emission Reduction in Fermentation Industry in Inner Mongolia,Hohhot 010050,China;Engineering Research Center of Inner Mongolia for Green Manufacturing in Bio-fermentation Industry,Hohhot 010050,China;School of Chemical Engineering,Inner Mongolia University of Technology,Hohhot 010050,China)

机构地区：[1]内蒙古自治区发酵产业节能减排工程技术研究中心,呼和浩特010050 [2]生物发酵绿色制造内蒙古自治区工程研究中心,呼和浩特010050 [3]内蒙古工业大学化工学院,呼和浩特010050

出　　处：《生物学杂志》2023年第3期91-95,共5页Journal of Biology

基　　金：内蒙古自治区科技重大专项(2019ZD021)。

摘　　要：以发酵和淀粉制糖工业产生的高粗纤维含量玉米皮为原料,通过多菌种固态发酵,降低玉米皮中粗纤维含量,增加发酵产物中蛋白含量,制备菌体蛋白生物发酵饲料。根据底物特点和产物目标选择适宜菌种进行发酵组合,采用单因素试验设计探究菌种比例、接种量、发酵方式、糖蜜含量及氮源种类对发酵产物中粗蛋白、真蛋白和粗纤维的影响。结果表明:最佳菌种比例为枯草芽孢杆菌CGMCC 1.7417∶枯草芽孢杆菌CICC 10090∶酿酒酵母CICC 32236∶产朊假丝酵母CGMCC 2.2878等于1∶1∶2∶2;种子液接种量为15%;最佳发酵方式为浅盘发酵;最佳糖蜜含量为10°Brix;最佳氮源种类为硫酸铵。玉米皮经多菌种固态发酵后,粗蛋白含量为21.38%,提升率32.47%;真蛋白含量为10.77%,提升率50.42%;粗纤维含量为17.78%,降低率14.19%。The high crude fiber content of corn husk produced by fermentation and starch and sugar industry was used as raw material,and the content of crude fiber in corn husk was reduced and the content of protein in fermentation products was increased by multi-strain solid state fermentation to prepare cell protein feed.Strains were selected for fermentation combinations according to the characteristics of substrates and product.And the effects of strain ratio,inoculation amount,fermentation method,molasses content and nitrogen source type on crude protein,crude fiber and true protein in fermentation products were investigated by single factor experimental design.The results showed that the optimum strain ratio of bacillus subtilis CGMCC 1.7417∶Bacillus subtilis CICC 10090∶Saccharomyces cerevisiae CICC 32236∶candida utilis CGMCC 2.2878 was 1∶1∶2∶2.The Inoculation amount of seed liquid was 15%.The best fermentation method was shallow plate fermentation.The optimum molasses content was 10°Brix.The best nitrogen source was ammonium sulfate.After multi-strain solid state fermentation,the crude protein content of product was 21.38% with an increase rate of 32.47%.The true protein content was 10.77% with an improvement rate of 50.42%.The crude fiber content was 17.78% with a reduction rate of 14.19%.

关 键 词：多菌种固态发酵 玉米皮 粗蛋白 真蛋白 粗纤维 

分 类 号：Q939.9[生物学—微生物学] S816.4[农业科学—饲料科学]