水稻OsRLCK167基因的组织表达模式和生物信息学分析  

Tissue Expression Pattern and Bioinformatics Analysis of OsRLCK167 Gene in Rice

作　　者：马银花 刘桃梅 万天雪 肖新波 胡琼 李萍芳 段仁燕 张斌 金晨钟 杜波 Ma Yinhua;Liu Taomei;Wan Tianxue;Xiao Xinbo;Hu Qiong;Li Pingfang;Duan Renyan;Zhang Bin;Jin Chenzhong;Du Bo(College of Agriculture and Biotechnology,Hunan University of Humanities,Science and Technology,Loudi 417000,China;College of Life Sciences,Wuhan University/National Key Laboratory of Hybrid Rice,Wuhan 430072,China)

机构地区：[1]湖南人文科技学院农业与生物技术学院,湖南娄底417000 [2]武汉大学生命科学学院/杂交水稻国家重点实验室,湖北武汉430072

出　　处：《山东农业科学》2023年第5期8-14,共7页Shandong Agricultural Sciences

基　　金：湖南省重点研发计划项目(2021NK2030);湖南省科技创新人才计划大学生科技创新创业项目(2021RC1016);湖南省自然科学基金面上项目(2021JJ30376);湖南省教育厅重点项目(21A0552);湖南省区域联合基金项目(2023JJ50084);杂交水稻国家重点实验室(武汉大学)开放基金项目(KF202207)。

摘　　要：类受体胞质激酶(receptor-like cytoplasmic kinases,RLCKs)家族是一类特殊蛋白激酶,在植物生长和病原菌防御中发挥着重要的生物学功能。在对水稻RLCKⅥ家族成员OsRRK1基因的研究中发现水稻4号染色体上存在一个与其同源性特别高的基因,即OsRLCK167(LOC_Os04g56060)。为了丰富类受体胞质激酶家族并探究水稻胞质受体激酶基因的作用,本研究利用ExPASy-Protparam、Protscale、CD-search等在线工具和DNAMAN软件对OsRLCK167基因进行生物信息学分析;采用qRT-PCR技术对OsRLCK167基因做组织表达模式分析。结果显示:OsRLCK167蛋白的分子量为43.77776 kD,为疏水性、不稳定的酸性蛋白;对该蛋白的二级结构进行预测,显示其主要由无规则卷曲(41.94%)、α-螺旋(35.29%)、延伸链(15.35%)和β-转角(7.42%)组成;该基因在水稻不同组织均有表达,且在叶鞘中的表达量最高。结果表明,OsRLCK167基因在进化过程中具有高度的保守性,在水稻中具有功能多样性。Receptor⁃like cytoplasmic kinases(RLCKs)are a special class of protein kinases which play important biological roles in plant growth and pathogen defense.In the research of the OsRRK1 gene,a mem⁃ber of the rice RLCKⅥfamily,a gene on rice chromosome 4 was found with particularly high homology to it,namely OsRLCK167(LOC_Os04g56060).In order to enrich the receptor cytoplasmic kinase family and explore the role of rice cytoplasmic receptor kinase gene,the bioinformatics analysis of OsRLCK167 was conducted with online tools such as ExPASy⁃Protparam,Protscale,CD⁃search and DNAMAN software,and its tissue ex⁃pression pattern was analyzed by qRT⁃PCR.The results showed that the molecular weight of OsRLCK167 pro⁃tein was 43.77776 kD,and it was a hydrophobic and unstable acidic protein.The prediction of its secondary structure showed that it was mainly composed of random curl(41.94%),α⁃helix(35.29%),extended chain(15.35%)andβ⁃turn(7.42%).The gene could express in different tissues of rice,and the expression level in leaf sheath was the highest.All these indicated that the OsRLCK167 gene was highly conserved in the evolu⁃tion process and had functional diversity in rice.

关 键 词：水稻 OsRLCK167基因 组织表达模式 生物信息学分析 

分 类 号：S511.01[农业科学—作物学] Q78[生物学—分子生物学]