TRX和TXNIP在结直肠癌组织中的表达及其交互作用  

Expression and interaction of TRX and TXNIP in colorectal cancer

作　　者：杨婕琳[1] 王晓媛 李海霞 YANG Jielin;WANG Xiaoyuan;LI Haixia(Department of Gastroenterology,the First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,Hebei,China)

机构地区：[1]河北北方学院附属第一医院消化内科,河北张家口075000

出　　处：《检验医学》2023年第4期330-336,共7页Laboratory Medicine

基　　金：2020张家口市级科技计划自筹经费项目(2021050D)。

摘　　要：目的探讨硫氧还蛋白(TRX)和硫氧还蛋白相互作用蛋白(TXNIP)在结直肠癌中的表达和交互作用。方法选取2019年1月—2020年6月河北北方学院附属第一医院行手术治疗的结直肠癌和直肠腺瘤患者各116例,收集所有患者的临床资料和术中留取的组织样本[癌组织、癌旁组织(距肿瘤下缘4~5 cm)和腺瘤(癌前病变)组织],采用免疫组化法检测3种组织样本中TRX和TXNIP的表达。采用免疫印迹法和实时荧光定量聚合酶链反应(RT-qPCR)检测3种组织样本中TRX、TXNIP蛋白和mRNA的相对表达量。采用广义多因子降维法(GMDR)评价TRX、TXNIP在结直肠癌中的交互作用。结果癌组织、癌前病变组织和癌旁组织之间TRX、TXNIP的阳性表达率差异均有统计学意义(P<0.05),TRX mRNA和蛋白的相对表达量依次降低(P<0.05),TXNIP mRNA和蛋白的相对表达量依次升高(P<0.05)。TRX阳性表达率在有无淋巴转移、不同浸润深度、不同癌胚抗原水平、不同TNM分期、不同肿瘤位置的结直肠癌患者之间差异均有统计学意义(P<0.05)。TXNIP阳性表达率在有无淋巴转移、不同临床分期、不同浸润深度、不同T分期、不同N分期的结直肠癌患者之间差异均有统计学意义(P<0.05)。GMDR交互作用分析结果表明,在结直肠癌中,TXNIP蛋白和mRNA低表达、TRX蛋白和mRNA高表达存在交互作用。结论TRX和TXNIP在结直肠癌中均呈异常表达,且可能在结直肠癌的发生、发展中发挥作用。Objective To investigate the interaction of thioredoxin(TRX)and thioredoxin-interacting protein(TXNIP)in colorectal cancer.Methods Totally,116 patients with colorectal cancer and 116 patients with rectal adenoma who underwent surgical treatment in the First Affiliated Hospital of Hebei North University from January 2019 to June 2020 were enrolled.The tissue samples[cancer tissue,adjacent tissue(4-5 cm from the lower edge of tumor)and adenoma tissue(precancerous lesion)]were collected,and the clinical data from all the patients were collected.The expressions of TRX and TXNIP in the 3 tissue samples were determined by immunohistochemistry.Immunoblotting and real-time fluorescence quantitative polymerase chain reaction(RTqPCR)were used to determine the relative expressions of TRX,TXNIP protein and mRNA in the 3 tissue samples.The interaction between TRX and TXNIP in colorectal cancer was evaluated by generalized multifactor dimensionality reduction(GMDR).Results The positive expression rates of TRX and TXNIP among cancer tissue,adenoma tissue and adjacent tissue had statistical significance(P<0.05).The relative expressions of TRX mRNA and protein were decreased in turn(P<0.05),and the relative expressions of TXNIP mRNA and protein were increased in turn(P<0.05).The positive expression rate of TRX had statistical significance among colorectal cancer patients with or without lymph metastasis,different depths of invasion,different levels of carcinoembryonic antigen,different TNM stages and different tumor locations(P<0.05).The positive expression rate of TXNIP had statistical significance among colorectal cancer patients with or without lymph metastasis,different clinical stages,different depths of invasion,different T stages and different N stages(P<0.05).GMDR interaction analysis showed that there was an interaction between low expressions of TXNIP protein and mRNA and high expressions of TRX protein and mRNA in colorectal cancer.Conclusions Both TRX and TXNIP are abnormally expressed in colorectal cancer,and they ma

关 键 词：硫氧还蛋白 硫氧还蛋白相互作用蛋白 结直肠癌 交互作用 

分 类 号：R446.1[医药卫生—诊断学]