子宫内膜癌组织miR-433的表达及其与化疗敏感性的关系  

Relation of miR-433 in endometrial cancer tissues and its relationship with chemotherapy sensitivity

作　　者：袁爽[1] 冯磊[1] 郭小芬[1] 付小玲[1] 许静[1] 刘弘扬[2] YUAN Shuang;FENG Lei;GUO Xiaofen;FU Xiaoling;XU Jing;LIU Hongyang(Department of Obstetrics and Gynecology,Nanyang Central Hospital,Nanyang 473000,Henan,China;Department of Obstetrics,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,Henan,China)

机构地区：[1]南阳市中心医院妇产科,河南南阳473000 [2]郑州大学第三附属医院产科,河南郑州450000

出　　处：《检验医学》2023年第4期337-341,共5页Laboratory Medicine

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20190354)。

摘　　要：目的探讨子宫内膜癌患者癌组织中微小RNA-433(miR-433)相对表达量与化疗敏感性的关系。方法选取2019年5月—2020年7月于南阳市中心医院确诊的子宫内膜癌患者98例,收集所有患者的临床资料,根据化疗4个周期后疗效是否敏感将所有患者分为化疗敏感组(79例)和化疗不敏感组(19例)。收集化疗结束后手术切除的癌组织和癌旁组织(距肿瘤边缘3 cm)。采用实时荧光定量聚合酶链反应(RT-qPCR)检测癌组织和癌旁组织miR-433相对表达量。采用多因素Logistic回归分析评估影响子宫内膜癌化疗敏感性的因素。结果子宫内膜癌患者癌组织miR-433相对表达量显著低于癌旁组织(P<0.05)。国际妇产科联合会(FIGO)分期Ⅲ~Ⅳ期、有淋巴转移、组织低分化的子宫内膜癌患者miR-433相对表达量分别低于FIGO分期Ⅰ~Ⅱ期、无淋巴转移、组织中/高分化的子宫内膜癌患者(P<0.05)。化疗不敏感组癌组织miR-433相对表达量显著低于化疗敏感组(P<0.05)。肿瘤直径、FIGO分期、淋巴转移、组织分化程度与子宫内膜癌化疗敏感性有关(P<0.05)。多因素Logistic回归分析结果显示,FIGO分期和miR-433相对表达量是影响子宫内膜癌化疗敏感性的危险因素[比值比(OR)值分别为2.716、0.847,95%可信区间(CI)分别为1.420~5.196、0.766~0.936]。结论miR-433在子宫内膜癌组织中呈低表达,与化疗敏感性有关。Objective To investigate the expression level of microRNA-433(miR-433)in patients with endometrial cancer and its relationship with chemotherapy sensitivity.Methods From May 2019 to July 2020,98 endometrial cancer patients in Nanyang Central Hospital were enrolled as endometrial cancer group.The clinical data were collected.According to the determination of sensitivity after 4 cycles of chemotherapy,the patients with endometrial cancer were classified into sensitive group(79 cases)and insensitive group(19 cases).Tumor tissues and paracancer tissues(3 cm from the edge of tumors)removed after chemotherapy were collected.Realtime fluorescence quantitative polymerase chain reaction(RT-qPCR)was used to determine the expression level of miR-433.Multiple Logistic regression analysis was used to analyze the factors affecting the chemotherapy sensitivity of endometrial cancer.Results Compared with adjacent tissue,the expression level of miR-433 in endometrial cancer tissue was lower(P<0.05).The expression level of miR-433 in patients with the International Federation of Gynecology and Obstetrics(FIGO)stageⅢ-Ⅳ,lymph metastasis and low differentiated endometrial cancer was lower than that in patients with FIGO stageⅠ-Ⅱ,non-lymph metastasis and medium/high differentiated endometrial cancer(P<0.05).Compared with sensitive group,the expression level of miR-433 in insensitive group was decreased(P<0.05).Tumor diameter,FIGO stage,lymph metastasis and the degree of tissue differentiation were related to chemotherapy sensitivity(P<0.05).Multiple Logistic regression analysis showed that FIGO stage and miR-433 relative expression level were risk factors affecting the chemotherapy sensitivity of endometrial cancer[odds ratios(OR)were 2.716 and 0.847,95%confidence intervals(CI)were 1.420-5.196 and 0.766-0.936,respectively].Conclusions MiR-433 is low-expressed in endometrial cancer tissues,which is a factor affecting chemotherapy sensitivity.

关 键 词：微小RNA-433 子宫内膜癌 化疗敏感性 

分 类 号：R446.1[医药卫生—诊断学]