基于线粒体控制区D-loop序列的洪泽湖翘嘴鲌遗传多样性分析  

Genetic Diversity Analysis of Culter alburnus in Hongze Lake Based on Mitochondrial Control Region D-loop Sequence

作　　者：杨子萍 李大命 刘燕山 谷先坤 杨家新 Yang Ziping;Li Daming;Liu Yanshan;Gu Xiankun;Yang Jiaxin(College of Marine Science and Engineering,Nanjing Normal University,Nanjing 210023,China;Freshwater Fisheries Research Institute of Jiangsu Province,Key Laboratory of Fisheries Resources in Inland Waters of Jiangsu Province,Nanjing 210017,China)

机构地区：[1]南京师范大学海洋科学与工程学院,江苏南京210023 [2]江苏省淡水水产研究所,江苏省内陆水域渔业资源重点实验室,江苏南京210017

出　　处：《南京师大学报（自然科学版）》2023年第2期70-76,共7页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：江苏省农业科技自主创新项目(CX(21)3003);2021和2022年度省级单位项目(2021-SJ-110-03,2022-SJ-061-02).

摘　　要：为掌握洪泽湖翘嘴鲌(Culter alburnus)种质资源遗传多样性状况,采集洪泽湖6个群体共233尾翘嘴鲌,采用线粒体控制区D-loop全序列进行遗传多样性分析.结果显示,翘嘴鲌线粒体D-loop序列长度为934 bp~936 bp,碱基(A+T)含量(66.0%)明显高于(G+C)含量(34.0%).243条D-loop序列检出23个变异位点,其中有9个单一信息位点,14个简约信息位点.6个群体发现22个单倍型,全部群体的单倍型多样性(H_(d))和核苷酸多样性(P_(i))分别为(0.794±0.019)和(0.00251±0.00014),呈现出高H_(d)和低P_(i)的遗传多样性模式.6个群体中洪泽湖群体的遗传多样性最高(Hd:0.873±0.038,Pi:0.00295±0.00035),顾勒河群体的遗传多样性最低(H_(d):0.709±0.064,P_(i):0.00203±0.00031).分子方差分析表明,群体间的分子变异占比0.43%,群体内的分子变异占比99.57%,分子变异主要发生在群体内.群体间遗传分化指数F_(st)为-0.01912~0.02801(P>0.05),表明群体间没有出现遗传分化.单倍型系统进化树和单倍型网络结构图显示,群体间拥有多个共享单倍型,单倍型聚为一个进化分支,没有形成特定的地理遗传结构.中性检验结果表明洪泽湖翘嘴鲌群体没有经历显著种群扩张事件.研究结果表明,洪泽湖翘嘴鲌遗传多样性较低,6个群体间基因交流频繁,遗传分化不显著,可将6个群体作为一个管理单元进行保护,需采取措施提高翘嘴鲌遗传多样性及恢复其种群数量.In order to understand the present genetic diversity of Culter alburnus populations in Hongze Lake,we sampled a total of 233 C.alburnus samples from 6 geographic populations and conducted genetic diversity analysis based on sequences of D-loop control region.The results showed that the full length of D-loop sequences was 934 bp-936 bp,(A+T)base composition(66.0%)was higher than the(C+G)combination(34.0%).A total of 23 variable sites were identified in 243 D-loop sequences in which 9 singleton mutation and 14 parsimony informative sites.22 haplotypes were defined in 6 populations.The overall haplotype diversity index(H_(d))was(0.794±0.019)and the overall nucleic acid diversity index(P_(i))was(0.00251±0.00014),which showed high H_(d) and lowπgenetic pattern.The highest and lowest index value of haplotype diversity and nucleic acid diversity were identified in Hongze popluation(H_(d):0.873±0.038,P_(i):0.00295±0.00035)and Gule River population(H_(d):0.709±0.064,P_(i):0.00203±0.00031),respectively.The result of AMOVA demonstrated that 0.43%molecular variation was among populations and 99.57%within populations,and molecular variation mainly occurred within populations.The values of pairwise genetic differentiation index were-0.01912-0.02801,which revealed that there were no sigificant genetic differences among pouplations(P>0.05).The NJ phylogenetic tree and network structure diagram of haplotypes showed that there were more shared haplotypes among populations,and haplotypes were clustered into one clade without forming a specific geographic genetic structure.The results of the neutrality test revealed that no population expansion happened in evolutionary history of C.alburnus.In conclusion,the genetic diversity of C.alburnus was low,and the 6 populations can be viewed as a management unit.Furthermore,we should take measures to improve its genetic diversity and population size of C.alburnus in Hongze Lake.

关 键 词：翘嘴鲌 控制区序列 遗传多样性 洪泽湖 

分 类 号：S917[农业科学—水产科学]