正交试验法优选通脉胶囊提取工艺  

Optimization of extraction process of Tongmai capsule by orthogonal test

作　　者：王璐 付玉琪 孙永慧[1] WANG Lu;FU Yuqi;SUN Yonghui(Institute of Traditional Chinese Medicine,Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学中医药研究院,哈尔滨150040

出　　处：《哈尔滨商业大学学报（自然科学版）》2023年第3期268-272,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

基　　金：黑龙江省自然科学青年基金(QC2018118)。

摘　　要：优选通脉胶囊的最佳提取工艺,以提取液中含黄芪甲苷的量和出膏率为评价指标,采用正交试验法设计并优化了通脉胶囊最佳提取工艺.黄芪甲苷测定的色谱条件为:Diamonsil C_(18)色谱柱(内径4.6 mm,柱长25 cm,粒度5μm);采用水∶乙腈(68∶32)为流动相;流速:1.0 mL/min;蒸发光散射检测器检测条件:空气气体流速2.5 L/min,漂移管温度105℃.实验结果表明,通脉胶囊的最佳提取工艺为12倍量水,提取2次,第一次2 h,第二次1.5 h.本实验确定的工艺合理可行,为通脉胶囊提取工艺的确定提供了实验依据.Optimum of the extraction process of Tongmai capsule.With the content of astragaloside A in the extraction solution and the extraction rate as evaluation indexes,the optimum extraction process of Tongmai capsule was designed and optimized by orthogonal test.The chromatographic conditions for the determination of astragaloside A content were:Diamonsil C_(18)chromatographic column(inner diameter 4.6 mm,column length 25 cm,particle size 5μm);water∶acetonitrile(68∶32)was used as mobile phase;flow rate:1.0 mL/min;detection conditions of evaporative light scattering detector:air gas flow rate 2.5 L/min,drift tube temperature 105℃.The experimental results showed that the best extraction process of Tongmai capsule was 12 times of water,twice,2 h for the first time;1.5 h for the second time.The process determined in this experiment was reasonable and feasible,which provided an experimental basis for the determination of the extraction process of Tongmai capsule.

关 键 词：正交试验 通脉胶囊 提取工艺 黄芪甲苷 含量测定 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]