短时两次取精精子动态参数的差异性分析  

Difference analysis of sperm dynamic parameters after sperm extraction twice in a short time

作　　者：刘永杰 石冬梅 代良 张帆 王国平 LIU Yongjie;SHI Dongmei;DAI Liang;ZHANG Fan;WANG Guoping(Reproductive Center,Maternal and Child Health Hospital of Yinchuan,Yinchuan 750001,China)

机构地区：[1]银川市妇幼保健院生殖中心,750001

出　　处：《天津医药》2023年第6期637-641,共5页Tianjin Medical Journal

基　　金：宁夏自然科学基金项目(2022AAC03744);宁夏重点研发计划项目(2022BEG03083);银川市科技计划项目(2021-SF-008)。

摘　　要：目的探讨短时两次取精精子动态参数的差异性。方法48例健康体检男性,要求禁欲2~7 d,手淫取精液于无菌杯中,在第1次取精后1 h,再次手淫取精液于无菌杯中。精液液化后,采用计算机辅助精子分析系统行精液分析,获得精液量、精子浓度、前向运动精子比例、直线运动精子比例;精子动态参数:平均曲线运动速度(VCL)、平均直线运动速度(VSL)、平均路径运动速度(VAP)、精子平均侧摆幅值(ALH)、精子平均鞭打频率(BCF)、精子平均移动角度(MAD)、运动的直线性(LIN)、运动的摆动性(WOB)、运动的前向性(STR);采用5-Br-PAPS法检测精浆锌含量;采用己糖激酶法检测精浆果糖含量;采用改良Cooper法检测精浆α-葡萄糖苷酶含量;分析两次取精上述指标差异及精液量、精子浓度、前向运动精子比例与精子各动态参数的关系。结果第1次取精的精液量、直线运动精子比例、精子总数、前向运动精子总数、直线运动精子总数、VCL、VAP、BCF、精浆锌及α-葡萄糖苷酶水平均高于第2次取精精液(P<0.05);两次精液的精子浓度、前向运动精子比例、VSL、ALH、MAD、LIN、WOB、STR和精浆果糖水平差异无统计学意义。第1次取精的精液量与BCF呈正相关,第2次取精的精液量与VCL、VSL、VAP、ALH、MAD、WOB呈负相关(P<0.05);第1次取精的精子浓度与BCF呈正相关,第2次取精的精子浓度与所有精子的动态参数均呈正相关(P<0.05);除第1次取精的BCF外,总体、第1次及第2次取精前向运动精子比例与其余精子的动态参数均呈正相关(P<0.05);总体和第1次精液的精浆锌、精浆果糖及α-葡萄糖苷酶均与BCF呈正相关(P<0.05);第2次精液的精浆果糖与BCF呈正相关,精浆α-葡萄糖苷酶与VCL和VAP呈负相关(P<0.05)。结论虽然短时第2次所取精液中精子的运动能力较第1次明显下降,但并不影响活动精子与卵子的结合能力,可以作为体外受精的有效补充Objective To investigate the difference of sperm dynamic parameters in two-time spermatozoa.Methods Forty-eight men who prepared for physical examination were selected in this study.The semen of them were collected after 1 h verses 2-7 days of ejaculation abstinence.Semen volume,sperm concentration,proportion of forward motile sperm and proportion of straight motile sperm were analyzed by computer-aided sperm analysis system.Sperm dynamic parameters included mean curve motion velocity(VCL),mean linear motion velocity(VSL),mean path motion velocity(VAP),mean sperm yaw amplitude(ALH),mean sperm whig frequency(BCF),mean sperm motion angle(MAD),straightness of motion(LIN),wobble of motion(WOB)and forward motion(STR).The plasma zinc content was determined by 5-Br-PAPS method.Fructose content was determined by hexokinase method.The content ofα-glycosidase in seminal plasma was determined by improved Cooper method after liquefaction.The differences of above indexes and the relationship between semen volume,sperm concentration,the proportion of forward motile sperm and the dynamic parameters of sperm were analyzed.Results The semen volume,proportion of linear motility sperm,total sperm,total forward motility sperm,total linear motility sperm,VCL,VAP,BCF,seminal plasma zinc andα-glucosidase levels of the first sperm extraction were higher than those of the second sperm extraction(P<0.05).There were no significant differences in sperm concentration,forward motility sperm ratio,VSL,ALH,MAD,LIN,WOB,STR and spermatoglycose level between sperm from two times of sperm extraction.The semen volume of the first extraction was positively correlated with BCF,and the semen volume of the second extraction was negatively correlated with VCL,VSL,VAP,ALH,MAD and WOB(P<0.05).The sperm concentration of the first sperm extraction was positively correlated with BCF,and the sperm concentration of the second sperm extraction was positively correlated with the dynamic parameters of all sperm(P<0.05).Except the BCF of the first sperm extractio

关 键 词：精子 精液分析 生育力 精子能动性 禁欲时间 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]