裂殖壶藻SlMYB118基因的克隆及生物信息学分析  

Cloning and bioinformatics analysis of SlMYB 118 gene from Schizochytrium limacinum SR 21

作　　者：陈菁 苏永昌[4] 孙化淼 陈由强[1,2,3] 薛婷 代容春[1,2,3] CHEN Jing;SU Yongchang;SUN Huamiao;CHEN Youqiang;XUE Ting;DAI Rongchun(College of Life Science,Fujian Normal University,Fuzhou 350117,China;The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration,Fuzhou 350117,China;Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization,Fuzhou 350117,China;Fisheries Research Institute of Fujian,Xiamen 361013,China)

机构地区：[1]福建师范大学生命科学学院,福建福州350117 [2]海洋生物医药与制品产业化开发技术公共服务平台,福建福州350117 [3]福建省特色海洋生物资源可持续利用重点实验室,福建福州350117 [4]福建省水产研究所,福建厦门361013

出　　处：《渔业研究》2023年第3期222-232,共11页Journal of Fisheries Research

基　　金：福建省自然科学基金计划项目(2020J01178)。

摘　　要：裂殖壶藻是一种含有丰富DHA的海洋微藻。本课题组前期发现裂殖壶藻的SlMYB118基因与脂肪酸的合成密切相关,是潜在的DHA合成调控的转录因子。本文通过PCR的方法克隆裂殖壶藻的SlMYB118基因的cDNA全长序列,运用生物信息学的方法对其分析。结果预测SlMYB118蛋白质的等电点为8.75,分子量为84.81 kDa,定位于细胞核,且其具有106个潜在的磷酸化位点。SlMYB118基因的结合位点是探索转录调控机制、建立DHA合成相关转录调控网络的关键,本研究为后续确定其结合位点奠定了理论基础。Schizochytrium limacinum SR21 is a kind of marine microalgae rich in DHA.The research group that the author joined previously found that SlMYB118 gene of S.limacinum SR21 was closely related to fatty acid synthesis and was a potential transcription factor for DHA synthesis regulation.In this paper,the full-length cDNA sequence of SlMYB118 gene was cloned by PCR and analyzed by bioinformatics method.The results predicted that SlMYB118 protein had an isoelectric point of 8.75,a molecular weight of 84.81 kDa,was localized in the nucleus,and had 106 potential phosphorylation sites.This study laid the theoretical foundation for the subsequent identification of the binding site of SlMYB118 gene,which was a key issue for the transcriptional regulatory mechanism and the establishment of the transcriptional regulatory network related to DHA synthesis.

关 键 词：裂殖壶藻 SlMYB118基因 克隆 生物信息学分析 

分 类 号：Q943.2[生物学—植物学]