养殖水体中甲苯咪唑的动态消除规律研究  

Study on the dynamic elimination pattern of mebendazole in aquaculture water

作　　者：钱卓真[1] 李雷斌[1] 刘智禹[1] 汤水粉[1] 王丽娟[1] 姜琳琳[1] 陈宇锋[1] 位绍红[1] 余颖[1] 刘海新[1] 陈思 罗方方[1] JIANG Linlin;CHEN Yufeng;WEI Shaohong;YU Ying;LIU Haixin;CHEN Si;LUO Fangfang(Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province,National and Local Joint Engineering Research Center of Marine Biological Seed Industry Technology,Fisheries Research Institute of Fujian,Xiamen 361013,China)

机构地区：[1]福建省水产研究所,福建省海洋生物增养殖与高值化利用重点实验室,海洋生物种业技术国家地方联合工程研究中心,福建厦门361013

出　　处：《渔业研究》2023年第3期297-303,共7页Journal of Fisheries Research

基　　金：福建省海洋与渔业结构调整专项(2021HYJG02);福建省省属公益类科研院所基本科研专项(2020R1013002)。

摘　　要：本文建立了高效液相色谱-串联质谱定量检测养殖水体中甲苯咪唑方法,方法定量限为0.5μg/L,3个添加水平的平均回收率为79.0%~93.9%,日内相对标准偏差(RSD)为7.57%~8.27%,日间RSD为7.43%~8.11%;分别以实际施药浓度15、50μg/L作为受试浓度,开展室外水体中甲苯咪唑的消除实验。结果表明,室外养殖水体在自然光照下,甲苯咪唑的光降解速率遵循一级动力学规律,648 h后甲苯咪唑的光降解率可达80%以上;在15~18℃水温下,水体中甲苯咪唑消除半衰期为231.05 h;实验结束(648 h)时,施药浓度为50μg/L的高浓度组水体中仍有6.4μg/L甲苯咪唑。本研究结果可为水产养殖动物中甲苯咪唑的药代动力学研究提供基础数据。A high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method was developed for the quantitative determination of mebendazole in aquaculture water.The limit of quantification was 0.5μg/L.The average recoveries of the three spiked levels ranged from 79.0%to 93.9%.The intra-day and inter-day relative standard deviations(RSDs)were 7.57%-8.27%and 7.43%-8.11%,respectively.The actual application concentrations of 15μg/L and 50μg/L were used as the tested concentrations for the elimination experiments of mebendazole in aquaculture water,respectively.The results showed that mebendazole was a photosensitive drug whose photodegradation followed the first-order kinetics.The photolytic degradation rate of mebendazole in outdoor aquaculture water under natural light was more than 80%after 648 h.The elimination half-life of mebendazole was 231.05 h at 15-18℃.At the end of the experiment(648 h),6.4μg/L of mebendazole was still detected in the high concentration group(50μg/L).Therefore,the results of this study could provide basic data for the pharmacokinetic study of mebendazole in aquaculture animals.

关 键 词：养殖水体 甲苯咪唑 光解 动态消除 

分 类 号：S94[农业科学—水产养殖]