低温胁迫对军曹鱼幼鱼鳃组织抗氧化能力、细胞凋亡和组织结构的影响  

Effect of low temperature stress on antioxidant stress,apoptosis and histological structure of gills in cobia(Rachycentron canadum)

作　　者：李豫 黄建盛[1] 陈有铭 温震威 欧光海 黄鉴鹏 蒋鑫涛 谢瑞涛 马骞 陈刚[1,4] LI Yu;HUANG Jiansheng;CHEN Youming;WEN Zhenwei;OU Guanghai;HUANG Jianpeng;JIANG Xintao;XIE Ruitao;MA Qian;CHEN Gang(College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China;Guangdong Blue Granary Seed Industry Co.,Ltd.,Zhanjiang 524256,China;Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture,Zhanjiang 524088,China;Guangdong Evergreen Feed Industry Co.Ltd.,Zhanjiang 524094,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]广东蓝粮种业有限公司,广东湛江524256 [3]广东省水产动物病害防控与健康养殖重点实验室,广东湛江524088 [4]广东恒兴饲料实业股份有限公司,广东湛江524094

出　　处：《南方水产科学》2023年第3期68-77,共10页South China Fisheries Science

基　　金：国家重点研发计划项目(2022YFD2401203,2020YFD0900200);广东省科技计划项目(2016B020201009);国家现代农业产业技术体系(CARS-47)。

摘　　要：军曹鱼(Rachycentron canadum)鳃组织对水温变化敏感。为探究低温胁迫对军曹鱼幼鱼鳃组织的影响,揭示该鱼应对低温胁迫的响应机制,实验设置2个低温胁迫组(18℃、21℃)和1个对照组(28℃),比较分析鳃组织在胁迫后第0、第4和第7天的氧化应激状态、细胞凋亡和组织结构情况。结果显示,低温胁迫下鳃组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性显著低于对照组(P<0.05),丙二醛(MDA)浓度显著高于对照组(P<0.05);低温胁迫组鳃组织凋亡相关基因bax、caspase-9、caspase-3、p53和mdm2表达量在第4和第7天时相较于对照组显著升高(P<0.05),bcl-2基因表达量显著降低(P<0.05);TUNEL检测显示低温组鳃组织细胞凋亡率升高;组织学分析表明低温胁迫下鳃组织出现了不同程度的鳃小片排列紊乱、基部增生、融合,上皮细胞和泌氯细胞空泡化等现象。研究表明,低温胁迫抑制了军曹鱼幼鱼的鳃组织抗氧化酶活性,造成氧化损伤,进一步诱导细胞凋亡,破坏鳃组织结构完整性。The gill tissue of cobia(Rachycentron canadum)is sensitive to water temperature changes.In order to investigate the effect of low temperature stress on juvenile cobia,and reveal its response mechanism to low temperature stress,we designed two low temperature groups(18℃and 21℃)and one control group(28℃),to analyze the antioxidant responses,expression of apoptosis-related genes and histological structure of the gills on 0,4th and 7th day after the stress.The results show that the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)in the low-temperature groups were significantly lower than those in the control group(P<0.05),while the malondialdehyde(MDA)mass concentration in the low-temperature groups was significantly higher than that in the control group(P<0.05).The expression of apoptosis-related genes bax,caspase-9,caspase-3,p53 and mdm2 increased significantly in the low-temperature groups,while the expression of Bcl-2 decreased significantly on 4th and 7th day(P<0.05).The TUNEL results reveal that low-temperature treatment increased the cell apoptosis rate of the gills,and caused lesions including fusion of secondary lamellae,necrosis of epithelial cell and hyperplasia of chloride cells.The results indicate that low-temperature stress causes oxidative stress,induces apoptosis and damages the structural integrity of the gills,which suggests that normal physiological functions of juvenile cobia can be affected by low temperature significantly.

关 键 词：军曹鱼 低温胁迫 鳃 氧化应激 细胞凋亡 组织结构 

分 类 号：Q178[生物学—水生生物学] S965.399[生物学—普通生物学]