紫外法快速检测青蒿素含量中的参比选择与优化  

Selection and Optimization of Reference in Rapid Determination of Artemisinin by UV Method

作　　者：唐利忠 孙小成 欧阳国春 谢宜芝 蒋艺 吴岳庭 朱旺冲 TANG Li-zhong;SUN Xiao-cheng;OUYANG Guo-chun;XIE Yi-zhi;JIANG Yi;WU Yue-ting;ZHU Wang-chong(Yongzhou Institute of Agricultural Sciences,Hunan Yongzhou 425100,China)

机构地区：[1]永州市农业科学研究所,湖南永州425100

出　　处：《广州化工》2023年第6期80-83,89,共5页GuangZhou Chemical Industry

基　　金：永州市指导性科技计划项目(永发科[2020]19号-20)。

摘　　要：通过确定并最大限度消除非青蒿素成分的吸收干扰,建立标准曲线等步骤,来优化样品吸光度值-浓度的线性关系。结果表明,青蒿素紫外检测波长为290~292 nm;以提取的样品溶液为基础,以立即加入0.2%NaOH溶液后摇匀后的反应溶液为参比液,能较为完全的消除非青蒿素成分的背景吸收;在0.00~0.20 mg·mL^(-1)范围内,青蒿素含量-吸光度值关系式为C(mg·mL^(-1))=0.156*Abs+0.009,R2=0.999;Bland-Altman一致性分析表明,紫外法与HPLC检测结果无显著差异(P(H0:mean=0)=0.848)。该方法稳定性、精密度和重复性较好。The linear relationship between absorbance value and concentration of samples was optimized by determining the optimal absorption wavelength of UV detection method,determining and eliminating the absorption interference of non artemisinin components to the greatest extent,and establishing standard curve.The results showed that the UV detection wavelength of artemisinin was between 290~292 nm.And the background absorption of non artemisinin components could be completely eliminated based on the extracted sample solution and the reaction solution shaken up immediately after adding 0.2%NaOH solution as the reference solution.The relationship between artemisinin content and absorbance value was C(mg·mL^(-1))=0.156A+0.009(R^(2)=0.999)in the wavelength range of 0.00~0.20 mg·mL^(-1).Bland-Altman consistency analysis showed that there was no significant difference between the detection results of UV method and HPLC(P(H0:mean=0)=0.848).The method had good stability,precision and repeatability.

关 键 词：青蒿素 特异性参比 乙醇提取 水浴加热 紫外检测 

分 类 号：O657.32[理学—分析化学]