滇重楼皂苷含量测定及累积规律研究  

Study on the Content Determination and Accumulation Law of Saponins in Paris polyphylla var.yunnanensis

作　　者：徐永艳[1,2] 李昆 焦蓉[3] 汪琼 王春涛[3] 郁步竹 徐荣 Xu Yongyan;Li Kun;Jiao Rong;Wang Qiong;Wang Chuntao;Yu Buzhu;Xu Rong(College of Landscape Architecture and Horticulture/Landscape Engineering Research Center in Southwest China,National Forestry and Grasseland Administraton,Southwest Forestry University,Kunming Yunnan 650233,China;Institute of Highland Forest Science,Chinese Academy of Forestry,Kunming Yunnan 650233,China;Yuxi Normal University,Yuxi Yunnan 653100,China)

机构地区：[1]西南林业大学园林园艺学院,国家林业和草原局西南风景园林工程技术研究中心,云南昆明650233 [2]中国林业科学研究院高原林业研究所,云南昆明650233 [3]玉溪师范学院,云南玉溪653100

出　　处：《西南林业大学学报（自然科学）》2023年第3期169-174,共6页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家林业公益性行业科研专项(201304202)资助;云南省基础研究项目(2020-530622-01-01-034399)资助;云南省地方本科高校基础研究联合专项(202101BA070001-055)资助;云南省高校玉溪特色农业资源发掘与利用重点实验室资助。

摘　　要：以7年生普洱景东种源滇重楼为材料,采用HPLC法测定滇重楼不同生长年限根茎,及茎、叶、花、种子等器官中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ含量,建立各部位的HPLC图谱;应用HPLC法测定1月、3月、5月、7月、9月、11月不同生长期滇重楼1年生根茎皂苷含量的变异规律。结果表明:滇重楼根部总皂苷含量显著高于其他器官;不同生长年限滇重楼根部总皂苷含量差异显著(P<0.05),皂苷含量从高到低排列为根茎2 a龄段、根茎3 a龄段、根茎4~5 a龄段、根茎1 a龄段、叶、种子、芽、茎、须根、果皮、花;非根茎部位中叶片总皂苷含量显著高于种子、果皮、茎等组织;不同生长季节总皂苷含量高低变化规律为1月>3月>5月、9月>7月、11月。因此,滇重楼根茎适宜在2 a龄、1—3月休眠季节进行采收。The contents of saponins I,II,VI and VII of the 7-year-old Paris polyphylla var.yunnanensis in Pu'er Jingdong were determined by HPLC.The content of saponins in different growth years of rhizome,stem,leaves,flowers and seeds were determined and the HPLC fingerprint of each part was established.The variation of 1-year-old rhizome saponin content of P.polyphylla var.yunnanensis in different growth periods in January,March,May,July,September and November was measured.The results show that the content of total saponins in rhizome was significantly higher than that in other organs.There were significant differences(P<0.05)in the content of saponins among different growth years rhizome.The saponin content from high to low as follows:2a rhizome,3a rhizome,4-5 a rhizome,1a rhizome,leaf,seed,bud,stem,fibrous root,pericarp and flower.The content of total saponins in leaves was significantly higher than that in seeds,pericarps,stems and other tissues except rhizomes.The variation of total saponin content in 1-age-old rhizomes of P.polyphylla var.yunnanensis in different growth seasons is January>March>May and September>July and November.It is suggested that the rhizome of should be harvested at the age of 2a and the dormancy period from January to March.

关 键 词：滇重楼 皂苷 高效液相色谱法 积累规律 

分 类 号：Q946[生物学—植物学]