基于HIV-1 pol基因DNA或RNA序列的分子传播网络的配对比较  

A paired comparison study of genetic transmission network base on HIV-1 pol gene DNA or RNA sequence

作　　者：陈金 陈欢欢[2] 罗柳红[2] 康瑞华 梁淑家[2] 朱秋映[2] 陆华湘[2] 刘秀 陈怡[2] 冯毅[1] 廖玲洁[1] 邢辉[1] 邵一鸣 蓝光华[2] 林玫[2] 宋畅 阮玉华[1] 李剑军[2] CHEN Jin;CHEN Huan-huan;LUO Liu-hong;KANG Rui-hua;LIANG Shu-jia;ZHU Qiu-ying;LU Hua-xiang;LIU Xiu;CHEN Yi;FENG Yi;LIAO Ling-jie;XING Hui;SHAO Yi-ming;LAN Guang-hua;LIN Mei;SONG Chang;RUAN Yu-hua;LI Jian-jun(National Center for AIDS/STD Control and Prevention(NCAIDS),Chinese Center for Disease Control and Prevention(China CDC),State Key Laboratory of Infectious Disease Prevention and Control(SKLID),Beijing 102206,China;Guangxi Center for Disease Control and Prevention,Guangxi Key Laboratory of Major Infectious Disease Prevention Control and Biosafety Emergency Response,Nanning,Guangxi 530028,China)

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心,传染病预防控制国家重点实验室,北京102206 [2]广西壮族自治区疾病预防控制中心,广西重大传染病防控与生物安全应急响应重点实验室,广西南宁530028

出　　处：《中国热带医学》2023年第5期443-448,共6页China Tropical Medicine

基　　金：国家科技重大专项(No.2018ZX10715008,No.2018ZX10721102-006-005);国家自然科学基金(No.82160636,No.82260670,No.11971479);广西自然科学基金项目(No.2020GXNSFAA159020);广西艾滋病防控与成果转化研究重点实验室(No.ZZH2020010);广西八桂学者艾滋病防控关键技术岗位专项。

摘　　要：目的 了解HIV感染者成对pol区DNA序列和RNA序列构建分子传播网络的差异,为利用DNA序列构建分子传播网络提供科学数据。方法 采集广西柳州2014年本底存活和2015—2018年新报告HIV感染者全血和血浆标本,分别进行DNA和RNA提取、序列扩增和序列测序、序列拼接、序列比对及亚型判定,构建DNA序列和RNA序列分子传播网络,并比较DNA序列构建的分子传播网络和RNA序列构建的分子传播网络的一致性。结果 本研究中2014年本底和2015—2018年新报告HIV感染者共2 983例,其中2014年本底仅有DNA序列2 590例、2014年本底和2015—2018年新报告的成对pol区DNA序列和RNA序列分别为196例和197例。在393例成对pol区DNA序列和RNA序列中,DNA序列和RNA序列的基因亚型一致,成对DNA序列和RNA序列的基因距离比较差异无统计学意义(Z=-2.72,P=1.00)。2015—2018年新报告HIV感染者成对pol区DNA序列和RNA序列与2014年本底DNA序列构建的分子传播网络连接一致性为91.4%(180/197);2015—2018年新报告HIV感染者成对pol区DNA序列和RNA序列与本底RNA序列构建的分子传播网络连接一致性为97.0%(191/197)。2015—2018年新报告HIV感染者成对pol区DNA序列和RNA序列分别与本底DNA序列构建分子传播网络,两组之间抗病毒治疗减少HIV二代传播的风险差异无统计学意义。结论 HIV感染者成对pol区DNA序列和RNA序列在亚型和基因距离及构建分子传播网络上具有很好的一致性,DNA序列和RNA序列均可用于分子传播网络分析。Objective To understand the difference of DNA sequence and RNA sequence of paired pol region in HIV patients in constructing HIV genetic transmission network,and to provide scientific data for constructing molecular transmission networks using DNA sequences.Methods The whole blood and plasma samples of HIV patients living in 2014 and newly reported in 2015-2018 in Liuzhou,Guangxi,were collected,DNA and RNA sequences were extracted,amplified,sequenced,spliced and aligned,and then genetic transmission networks were constructed,and the connectivity consistency of genetic transmission networks constructed by DNA and RNA sequences was compared.Results In this study,a total 2983 participants were investigated,which were 2014 baseline and 2015-2018 newly reported HIV patients,of which 2590 participants were only DNA sequences in 2014 baseline,196 HIV patients were both DNA and RNA sequences of paired pol region in 2014 baseline,and 197 newly reported HIV patients were both DNA and RNA sequences of paired pol region in 2015-2018.In 393 DNA and RNA sequences of paired pol region,the genotype of DNA sequence and RNA sequence were consistent,and there was no statistically significant difference in genetic distance between paired DNA sequences and RNA sequences(Z=-2.72,P=1.00).The connection consistency rate of genetic transmission networks constructed by DNA and RNA sequences of paired pol region of 2015-2018 newly reported with the baseline DNA sequences was 91.4%(108/197).And the connection consistency rate of genetic transmission networks constructed by DNA and RNA sequences of paired pol region of 2015-2018 newly reported with the baseline RNA sequences was 97.0%(191/197).There was no statistical difference in antiretroviral therapy to reduce the risk of HIV secondary transmission between genetic transmission networks constructed by DNA and RNA sequences of paired of pol gene newly reported from 2015 to 2018 with baseline DNA sequence.Conclusion DNA sequence and RNA sequence of paired pol region of HIV patients have goo

关 键 词：艾滋病病毒 DNA序列 RNA序列 分子传播网络 抗病毒治疗 

分 类 号：R512.91[医药卫生—内科学]