茄子叶色黄化突变体chl234的特性及其叶绿体超微结构  

The Characteristics and Chloroplast Ultrastructure of Yellow Leaf Mutant chl234 in Eggplant

作　　者：李淑培 张映[1] 舒金帅[1] 陈钰辉[1] 杨锦坤 陈露露 刘富中[1] LI Shupei;ZHANG Ying;SHU Jinshuai;CHEN Yuhui;YANG Jinkun;CHEN Lulu;LIU Fuzhong(State Key Laboratory of Vegetable Biobreeding,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081

出　　处：《园艺学报》2023年第5期1000-1008,共9页Acta Horticulturae Sinica

基　　金：中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS);农业农村部园艺作物生物学与种质创制重点实验室项目。

摘　　要：以茄子叶色黄化突变体chl234及其叶色正常的野生型为试材,比较发现:chl234从子叶期开始出现黄化现象,整个生育期真叶呈黄色,子叶变小,始花期延长,而始花节位、生长势、果实大小和种子大小与野生型无显著差异;在苗期、门茄开花期和四门斗开花期叶绿素含量显著降低;在苗期叶片净光合速率、气孔导度和蒸腾速率显著低于野生型,胞间CO_(2)浓度显著高于野生型;在四门斗开花期净光合速率、蒸腾速率和胞间CO_(2)浓度与野生型无显著差异,气孔导度显著低于野生型;chl234叶绿体内部基粒片层垛叠数较少,基粒排列不整齐。利用6个遗传分析群体对chl234叶色黄化性状进行遗传分析,结果表明该叶色黄化性状受隐性细胞核单基因控制。Yellow leaf eggplant mutant chl234 and its wild type were used as materials.chl234exhibited a yellow-leaf phenotype from cotyledon stage and the true leaves exhibited yellow existed throughout the life cycle.Compared with wild type,chl234 had smaller cotyledon and delayed flowering,and there was no significant difference in the first flower node,plant growth trend,fruit size and seed size between chl234 and wild type.The chlorophyll content in chl234 leaves was significantly lower than that in wild type at the seeding stage,first flowering stage and fourth flowering stage.The net photosynthetic rate,stomatal conductance and transpiration rate of chl234 were significantly lower than that of wild type at the seedling stage,while the intercellular CO_(2)concentration was significantly higher than wild type.The net photosynthetic rate,transpiration rate and intercellular CO_(2)concentration was no significant difference between chl234 and wild type,while stomatal conductance was significantly lower than that of wild type at fourth flowering stage.chl234 had less grana lamellae stacked pile and irregular arrangement of base grains.The inheritance of the leaf color yellowing trait of chl234 using six genetic analysis populations showed that the leaf color yellowing trait was controlled by a recessive nuclear single gene.

关 键 词：茄子 黄化突变体 叶绿素含量 光合特性 叶绿体超微结构 遗传分析 

分 类 号：S641.1[农业科学—蔬菜学]