血液中11种传统毒品及代谢物的液相色谱-串联质谱检验方法及应用  

Detection and application of 11 traditional drugs and metabolites in blood by liquid chromatography mass spectrometry

作　　者：武丽娜 施妍 向平 林佳曼 李天乐 贠克明[1] 刘耀[1,2] WU Li-na;SHI Yan;XIANG Ping;LIN Jia-man;LI Tian-le;YUN Ke-ming;LIU Yao(Department of Toxicology,College of Forensic Medicine,Shanxi Medical University,Jinzhong 030600,Shanxi Province,China;Material Evidence Identification Center of the Ministry of Public Security,Beijing 100038,China;Academy of Forensic Sciences-Shanghai Key Laboratory of Forensic Medicine-Shanghai Professional and Technical Service Platform of Forensic Medicine-Key Laboratory of Forensic Medicine,Ministry of Justice,Shanghai 200063,China)

机构地区：[1]山西医科大学法医学院毒化教研室,晋中030600 [2]公安部物证鉴定中心,北京100038 [3]司法鉴定科学研究院-上海市法医学重点实验室-上海市司法鉴定专业技术服务平台-司法部司法鉴定重点实验室,上海200063

出　　处：《复旦学报（医学版）》2023年第3期447-455,共9页Fudan University Journal of Medical Sciences

基　　金：司法部司法鉴定重点实验室资助项目;上海市法医学重点实验室项目(21DZ2270800);上海市司法鉴定专业技术服务平台资助项目(19DZ2292700);上海市人才发展项目(2020102);国家自然科学基金(82130056)。

摘　　要：目的建立同时分析血液中11种传统毒品及代谢物的液相色谱-串联质谱(LC-MS/MS)快速测定方法。方法血液经乙腈沉淀蛋白后用Phenomenex Kinetex Biphenyl 100Å(100 mm×3.0 mm×2.6µm)色谱柱分离,以11种毒品对应的氘代标品及甲氧那明作为内标,采用多反应监测模式同时测定血液中11种传统毒品及代谢物。结果各化合物检出限为0.5~2.0 ng/mL,定量限为1~5 ng/mL,在相应浓度范围内,11种传统毒品及代谢物均具有良好的线性关系,相关系数大于0.995,方法准确度为92.25%~109.56%,提取回收率为54.21%~101.86%,基质效应为79.60%~111.12%。结论该方法选择性强、灵敏度高、所需样品量少(100μL)、前处理过程简单快速(小于5 min),可用于毒驾等司法鉴定案件中11种传统毒品及代谢物的检验。Objective To establish a rapid method for the simultaneous analysis of 11 traditional drugs and metabolites in blood by liquid chromatography mass spectrometry(LC-MS/MS).Methods The blood was precipitated with acetonitrile and then separated with Phenomenex Kinetex Biphenyl 100Å(100 mm×3.0 mm×2.6µm)chromatographic column,with internal standard of methoxyphenamine and deuterated standard corresponding to 11 drugs.Eleven traditional drugs and metabolites in blood was detected by multi reaction monitoring mode simultaneously.Results The limit of detection of each compound was 0.5-2.0 ng/mL and the limit of quantitative was 1-5 ng/mL.Within the corresponding concentration range,11 traditional drugs and metabolites had good linear relationship,the correlation coefficient was greater than 0.995,the accuracy of the method was in the range of 92.25%-109.56%,the recoveries were in the range of 54.21%-101.86%,and the matrix effect was 79.60%-111.12%.Conclusion The method has strong selectivity,high sensitivity and characterized by a low required sample volume(100μL),a simple and fast pretreatment process(less than 5 min).It can be used to detect 11 traditional drugs and metabolites in forensic cases such as drug driving.

关 键 词：法医毒化 毒驾 液相色谱-串联质谱法 血液 传统毒品 代谢物 

分 类 号：R89[医药卫生—法医学]