LAMP耦合荧光侧向流层析试纸条的空间微生物快速检测技术  

Space Microbial Detection Method Based on Fluorescent LAMP

作　　者：韩培[1] 侯红渠 樊云龙 王文甲 吕雪飞[2] 张伟[1] 李晓琼[2] HAN Pei;HOU Hongqu;FAN Yunlong;WANG Wenjia;LÜXuefei;ZHANG Wei;LI Xiaoqiong(Center for Space Science and Applied Research,Chinese Academy of Sciences,Beijing 100094;School of Life,Beijing Institute of Technology,Beijing 100081)

机构地区：[1]中国科学院空间应用工程与技术中心,北京100094 [2]北京理工大学生命学院,北京100081

出　　处：《空间科学学报》2023年第2期302-309,共8页Chinese Journal of Space Science

基　　金：中国科学院空间应用工程与技术中心所长基金课题项目(Y903191);国防科技战略先导计划项目(20-ZLXD-21-03-02-002-03)共同资助。

摘　　要：微生物种类及其含量监测是空间站内微生物控制的重要环节。但是空间环境的微重力条件及对资源的条件限制导致地面常规检测实验难以开展,因此在轨微生物检测主要依靠培养法。基于侧流层析试纸条的生物分子识别检测方法具有不受微重力环境影响的优点,耦合荧光检测方法可以达到较高的检测灵敏度,是在轨微生物检测的潜在方法之一。针对空间环境中对航天员生活环境及仪器仪表设备具有潜在危害的微生物,研究了一种基于环介导等温扩增(LAMP)耦合荧光侧流层析试纸条的微生物核酸鉴别技术。研究结果表明,该技术可实现对大肠杆菌、金黄色葡萄球菌等空间站常见有害微生物的高灵敏、高特异性、快速检测,检测时间小于60 min,灵敏度达到100 copy·μL^(–1)。Relevant studies on the International Space Station have shown that during the long-term operation of the space station,the existence of microorganisms may pose a threat to the long-term operation of the space station and stay of astronauts.Microbial species and content monitoring are important parts of microbial control in the station.Due to resource constraints such as volume and power consumption in space applications,detection methods based on lateral flow chromatography test strips and supporting handheld devices will be more suitable for daily monitoring of space microorganisms,combined with loop-mediated isothermal amplification and up-conversion luminescence characteristic spectra.Nucleic acid identification technology can meet the needs of rapid,sensitive and specific identification of microorganisms.In this research,aiming at the potential harmful microorganisms in the space for the living environment of astronauts and instrumentation equipment,the nucleic acid identification technology based on fluorescent LAMP(Loop-mediated isothermal Amplification)of the lateral flow chromatography test strip was developed.Research results showed that this technology can achieve high sensitivity,high specificity and rapid detection of common harmful microorganisms on the space station such as Escherichia coli and Staphylococcus aureus.The detection time is less than 60 minutes and the sensitivity can reach 100 copy·μL^(–1).

关 键 词：空间微生物 侧流层析试纸条 环介导等温扩增 

分 类 号：V524[航空宇航科学与技术—人机与环境工程]