长期禁食对尿酸代谢的影响及其调控机制探讨  

Effect of Prolonged Fasting on Uric Acid Metabolism and Its Regulation Mechanism

作　　者：隋修锟 郭志峰[2] 张洪玉[2] 王海龙 吴峰[2] 杨超[2] 郭雅秀 马婷 李莹辉[2] 戴钟铨[2] SUI Xiukun;GUO Zhifeng;ZHANG Hongyu;WANG Hailong;WU Feng;YANG Chao;GUO Yaxiu;MA Ting;LI Yinghui;DAI Zhongquan(Department of Electronic and Information Engineering,Harbin Institute of Technology,Shenzhen 518055;State Key Laboratory of Space Medicine Fundamentals and Application,China Astronaut Research and Training Center,Beijing 100094;Space Science and Technology Institute(Shenzhen),Shenzhen 518003)

机构地区：[1]哈尔滨工业大学(深圳)电子与信息工程学院,深圳518055 [2]中国航天员科研训练中心航天医学基础与应用国家重点实验室,北京100094 [3]深圳市绿航星际太空科技研究院,深圳518003

出　　处：《空间科学学报》2023年第2期310-320,共11页Chinese Journal of Space Science

基　　金：深圳市科创委基础研究项目(JCYJ20200109110630285);中国航天员科研训练中心航天医学基础与应用国家重点实验室项目(SMFA17A02,SMFA18B02,SMFA18B06,SMFA19A01,SMFA19C03);飞天基金项目(2022SY54B0506)共同资助。

摘　　要：为研究长期禁食过程中大鼠尿酸代谢的变化及其潜在的调控机制,以Sprague-Dawley(SD)大鼠为动物模型,通过病理组织切片、生化检测、荧光定量PCR(qRT-PCR)以及蛋白免疫印迹(Western blotting)等方法分析不同禁食时间(1,2,3,5,7天)大鼠尿酸水平及其代谢相关基因和蛋白的表达变化。结果表明,长期禁食未对大鼠肾脏组织产生明显的损伤,引起了血尿酸水平上升、尿尿酸水平波动性变化和血液尿酸酶活性升高;随着禁食时间的延长,主要尿酸转运蛋白的mRNA和蛋白表达水平逐渐上调。长期禁食过程中大鼠尿酸代谢变化可能与尿酸转运蛋白及尿酸酶活性有关。To investigate the changes of uric acid metabolism in rats during prolonged fasting and its potential regulatory mechanism.Sprague-Dawley(SD)rats were used as animal models.The changes of uric acid level and metabolism-related gene and protein expression in rats during different fasting periods(1,2,3,5,and 7 days)were analyzed by pathological tissue sections,biochemical tests,quantitative PCR(qRT-PCR),and western blotting.The results showed that prolonged fasting did not cause significant damage to kidney tissue,but remarkedly increased the blood uric acid level and blood uricase activity.It also resulted in fluctuating changes in urine uric acid level.The mRNA and protein expression levels of main uric acid transporters were increased gradually with the fasting duration.The elevation of serum uric acid caused by prolonged fasting is related to uric acid transporter and uricase activity.

关 键 词：长期禁食 尿酸代谢 调控机制 低代谢 

分 类 号：V524[航空宇航科学与技术—人机与环境工程]