萱草品种‘六月花’与‘海尔范’杂交F 1代内外花被片颜色遗传分析  

Genetic analysis of the color of inheritance of internal and external tepals in the F 1 generation of the hy⁃brids between Hemerocallis cvs.‘Liu Yue Hua’and‘Frans Hals’

作　　者：秦钰乾 岳润 王金耀[1,2] 侯非凡 张伟 杨文静[1,2] 李森 邢国明 Qin Yuqian;Yue Run;Wang Jinyao;Hou Feifan;Zhang Wei;Yang Wenjing;Li sen;Xing Guoming(School of Horticulture,Shanxi Agricultural University,Jinzhong 030801,China;Datong Daylily Industrial Develop-ment Research Institute,Datong 037004,China)

机构地区：[1]山西农业大学园艺学院,山西晋中030801 [2]大同黄花产业发展研究院,山西大同037004

出　　处：《山西农业大学学报（自然科学版）》2023年第2期67-81,共15页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：山西省农业重点研发计划重点项目(201903D211011-01);山西农业大学青年拔尖创新人才支持计划(BJRC201601);山西农业大学科研项目生物育种工程(YZGC122);山西省研究生教育创新项目(2021Y321)。

摘　　要：[目的]萱草属植物具有重要的经济价值和观赏价值,而花色是其主要观赏性状之一,探析萱草属植物杂交后代内外花被片颜色的遗传规律可为培育新型花色品种提供理论依据。[方法]本研究以黄花菜单色花品种‘六月花’为母本,萱草双色花品种‘海尔范’为父本进行杂交构建了F1群体,采用分光测色计对杂交F1代的内、外花被片颜色进行测量并计算其色光值,基于内、外花被片的L^(*)、a^(*)、b^(*)值进行聚类分析并确定颜色分级,采用主基因+多基因混合遗传模型探究萱草属植物杂交F1代内、外花被片花色性状的遗传规律。[结果]萱草属植物杂交F1群体出现了黄色到深红色的花色分离,通过聚类分析将杂交F1群体内花被片划分为黄色、鲜肉色、淡珊瑚色、印度红、深红色5个色系,外花被片皆为黄色系,划分为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ5个等级;基于内、外花被片色光值和颜色分级进行遗传分析,确定内花被片颜色受2对主基因控制,遗传模型为2对加性-显性主基因模型(2MG-AD),第1对主基因加性效应更强,第2对主基因显性效应更强,主基因遗传率较高,为85.07%;确定外花被片颜色受2对主基因控制,遗传模型为2对加性-显性主基因模型(2MG-AD),且第1对主基因的加性效应和显性效应更强,主基因遗传率为36.05%。[结论]研究结果可为萱草属植物花被片颜色性状的基因定位和新型花色育种提供理论依据。[Objective]The daylily(Hemerocallis spp.)has important economic and ornamental values,and the flower color is one of its main ornamental traits.Analyzing the inheritance pattern of flower color in the hybrid progeny of Hemerocallis hy⁃brids can provide the theoretical basis for breeding new flower color varieties.[Methods]In this study,the single-colored yellow flower variety'Liu Yue Hua'was used as the maternal parent,and the bi-colored daylily variety'Frans Hals'was used as the pa⁃ternal parent to construct F1 population.The color values of the inner and outer tepals of the F1 generation were measured using a spectrophotometer,and the color was classified on the L*,a*and b*values of the inner and outer tepals using cluster analysis.The inheritance pattern of the flower color traits in the F1 generation of daylily hybrids was investigated using a major gene plus polygene mixed inheritance model.[Results]The results showed that the F1 population of daylily genus hybrids exhibited flower color segregation from yellow to deep red.The inner tepals of the F1 population were divided into five color series:yellow,salmon,light coral,Indian red,and dark red by cluster analysis,while the outer tepals were all in the yellow series,divided into five levels:Ⅰ,Ⅱ,Ⅲ,Ⅳ,andⅤ.The analysis of the color values and color grading of the inner and outer tepals revealed that the color of inner tepals was controlled by two pairs of master genes,and the genetic model was a two pairs additive-domi⁃nant master gene model(2MG-AD).The additive effect of the first pair of master genes was stronger,while the dominant ef⁃fect of the second pair of master genes was stronger.The genetic heritability of the master genes was higher than others,ac⁃counting for 85.07%.The color of the outer tepals was controlled by two pairs of master genes,and the genetic model was a two pairs additive-dominant master gene model(2MG-AD).The additive and dominant effects of the first pair of master genes were stronger,and the genetic heritability o

关 键 词：萱草属 内外花被片 颜色 表型变异 主基因+多基因 遗传分析 

分 类 号：S682[农业科学—观赏园艺]