低能量红光在不同细胞指标中的有效剂量筛选  

Effective dose screening of low-level red light in different cell indexes

作　　者：邱舒婷 潘志 胡璠 蒋晓 邬凤娟 张浩 QIU Shu-ting;PAN Zhi;HU Fan;JIANG Xiao;WU Feng-juan;ZHANG Hao(Shenzhen Synergy Testing Co.,Ltd.,Shenzhen,Guangdong 518055,China;Guangzhou Xingji Yuedong Co.,Ltd.,Guangzhou,Guangdong 510610,China;Guangzhou Juzi Youpin Co.,Ltd.,Guangzhou,Guangdong 510610,China)

机构地区：[1]深圳协同功效检测有限公司,广东深圳518055 [2]广州星际悦动股份有限公司,广东广州510610 [3]广州桔子有品生物科技有限公司,广东广州510610

出　　处：《日用化学品科学》2023年第6期29-36,共8页Detergent & Cosmetics

摘　　要：通过实验筛选在不同细胞指标上产生积极响应的低能量红光辐照剂量。选择成纤维细胞进行检测,筛选对线粒体膜电位、ATP释放量、细胞迁移率和胶原合成量4个指标产生积极响应的低能量红光辐照剂量。实验结果表明,2 J/cm^(2)的辐照剂量对成纤维细胞中的上述4个指标均可产生积极响应。将成纤维细胞上的测试模型迁移到毛乳头细胞中进行实验验证,结果表明2 J/cm^(2)的辐照剂量在毛乳头细胞的线粒体膜电位、ATP释放量、细胞增殖率和胶原合成量4个指标上有积极响应。由此证明,2 J/cm^(2)的辐照剂量可以提高成纤维细胞和毛乳头细胞的线粒体功能、细胞迁移率和胶原合成量,从而为细胞活动提供能量、提高细胞修复能力和细胞抗衰能力。The low-level red light irradiation dose that produced positive response on different cell indexes was screened through experiments.Fibroblasts were chosen for detection,and low-level red light irradiation dose that had positive response to the four indexes,i.e.,mitochondrial membrane potential,ATP release,cell migration and collagen synthesis,was screened.The experimental results show that the irradiation dose of 2 J/cm^(2) has positive response to the above four indexes in fibroblasts.The test model on fibroblasts was transferred to dermal papilla cells for experimental verification.The results show that the irradiation dose of 2 J/cm^(2) has positive response to the four indexes of dermal papilla cells,i.e.,mitochondrial membrane potential,ATP release,cell proliferation rate and collagen synthesis.Therefore,it is proved that the irradiation dose of 2 J/cm^(2) can improve the mitochondrial function,cell migration and collagen synthesis of fibroblasts and dermal papilla cells,thus providing energy for cell activities,improving cell repair ability and cell anti-aging ability.

关 键 词：低能量红光 剂量 线粒体功能 胶原合成 细胞迁移 

分 类 号：TQ658[化学工程—精细化工]